PGK1 as Predictor of CXCR4 Expression, Bone Marrow Metastases and Survival in Neuroblastoma

被引：20

作者：

Ameis, Helen M. ^{[1
]}

Drenckhan, Astrid ^{[2
]}

von Loga, Katharina ^{[3
]}

Escherich, Gabriele ^{[4
]}

Wenke, Katharina ^{[1
]}

Izbicki, Jakob R. ^{[2
]}

Reinshagen, Konrad ^{[1
]}

Gros, Stephanie J. ^{[2
]}

机构：

[1] Altona Childrens Hosp, Univ Med Ctr Hamburg Eppendorf, Dept Pediat Surg, Hamburg, Germany

[2] Univ Med Ctr Hamburg Eppendorf, Dept Gen Visceral & Thorac Surg, Hamburg, Germany

[3] Univ Med Ctr Hamburg Eppendorf, Dept Pathol, Hamburg, Germany

[4] Univ Med Ctr Hamburg Eppendorf, Dept Pediat Hematol & Oncol, Hamburg, Germany

来源：

PLOS ONE | 2013年 / 8卷 / 12期

关键词：

CHEMOKINE RECEPTOR CXCR4; PHOSPHOGLYCERATE KINASE 1; GASTRIC-CANCER; TUMOR-METASTASIS; PROSTATE-CANCER; PERITONEAL DISSEMINATION; NOD/SCID MICE; BREAST-CANCER; MOUSE MODEL; FACTOR-I;

D O I：

10.1371/journal.pone.0083701

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background and Aim: A close relationship between phosphoglycerate kinase 1 (PGK1) and the CXCR4/SDF1 axis (chemokine receptor 4/stromal cell derived factor 1) has been shown for several cancers. However, the role of PGK1 has not been investigated for neuroblastoma, and PGK1 might be a therapeutic target for this tumor entity. The aim of the current study was to evaluate the role of PGK1 expression in neuroblastoma patients, to determine the impact of PGK1 expression levels on survival, and to correlate PGK1 expression with CXCR4 expression and bone marrow dissemination. Materials and Methods: Samples from 22 patients with neuroblastoma that were surgically treated at the University Medical Center Hamburg-Eppendorf were evaluated for expression of PGK1 and CXCR4 using immunohistochemistry. Results were correlated with clinical parameters, metastases and outcome of patients. Immunocytochemistry, proliferation and expression analysis of CXCR4 and PGK1 were performed in neuroblastoma cell lines. Results: PGK1 is expressed in neuroblastoma cells. PGK1 expression is significantly positively correlated with CXCR4 expression and tumor dissemination to the bone marrow. Moreover the expression of PGK1 is significantly associated with a negative impact on survival in patients with neuroblastoma. PGK1 is downregulated by inhibition of CXCR4 in neuroblastoma cells. Conclusion: PGK1 appears to play an important role for neuroblastoma, predicting survival and tumor dissemination. Further in vivo studies outstanding, it is a candidate target for novel therapeutic strategies.

引用

页数：8

共 57 条

[1] CXCL12 does not attract CXCR4+ human metastatic neuroblastoma cells:: Clinical implications [J].

Airoldi, I ;

Raffaghello, L ;

Piovan, E ;

Cocco, C ;

Carlini, B ;

Amadori, A ;

Corrias, MV ;

Pistoia, V .

CLINICAL CANCER RESEARCH, 2006, 12 (01) :77-82

[2] The chemokine SDF-1 is a chemoattractant for human CD34(+) hematopoietic progenitor cells and provides a new mechanism to explain the mobilization of CD34(+) progenitors to peripheral blood [J].

Aiuti, A ;

Webb, IJ ;

Bleul, C ;

Springer, T ;

GutierrezRamos, JC .

JOURNAL OF EXPERIMENTAL MEDICINE, 1997, 185 (01) :111-120

[3] Suppression of neuroblastoma growth by dipeptidyl peptidase IV: relevance of chemokine regulation and caspase activation [J].

Arscott, W. T. ;

LaBauve, A. E. ;

May, V. ;

Wesley, U. V. .

ONCOGENE, 2009, 28 (04) :479-491

[4] REVISIONS OF THE INTERNATIONAL CRITERIA FOR NEUROBLASTOMA DIAGNOSIS, STAGING, AND RESPONSE TO TREATMENT [J].

BRODEUR, GM ;

PRITCHARD, J ;

BERTHOLD, F ;

CARLSEN, NLT ;

CASTEL, V ;

CASTLEBERRY, RP ;

DEBERNARDI, B ;

EVANS, AE ;

FAVROT, M ;

HEDBORG, F ;

KANEKO, M ;

KEMSHEAD, J ;

LAMPERT, F ;

LEE, REJ ;

LOOK, AT ;

PEARSON, ADJ ;

PHILIP, T ;

ROALD, B ;

SAWADA, T ;

SEEGER, RC ;

TSUCHIDA, Y ;

VOUTE, PA .

JOURNAL OF CLINICAL ONCOLOGY, 1993, 11 (08) :1466-1477

[5] Neuroblastoma: Biological insights into a clinical enigma [J].

Brodeur, GM .

NATURE REVIEWS CANCER, 2003, 3 (03) :203-216

[6] The International Neuroblastoma Risk Groups (INRG): a preliminary report [J].

Castleberry, RP ;

Pritchard, J ;

Ambros, P ;

Berthold, F ;

Brodeur, GM ;

Castel, V ;

Cohn, SL ;

De Bernardi, B ;

Dicks-Mireaux, C ;

Frappaz, D ;

Haase, GM ;

Haber, M ;

Jones, DR ;

Joshi, VV ;

Kaneko, M ;

Kemshead, JT ;

Kogner, P ;

Lee, REJ ;

Matthay, KK ;

Michon, JM ;

Monclair, R ;

Roald, BR ;

Seeger, RC ;

Shaw, PJ ;

Shimada, H ;

Shuster, JJ .

EUROPEAN JOURNAL OF CANCER, 1997, 33 (12) :2113-2116

[7]

CICCARONE V, 1989, CANCER RES, V49, P219

[8] Secretion of phosphoglycerate kinase from tumour cells is controlled by oxygen-sensing hydroxylases [J].

Daly, EB ;

Wind, T ;

Jiang, XM ;

Sun, L ;

Hogg, PJ .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2004, 1691 (01) :17-22

[9] Seeking a home for a PET, Part 2 - Defining the appropriate place for positron emission tomography imaging in the staging of patients with suspected lung cancer [J].

Detterbeck, FC ;

Falen, S ;

Rivera, MP ;

Halle, JS ;

Socinski, MA .

CHEST, 2004, 125 (06) :2300-2308

[10] Disease outcome may be predicted by molecular detection of minimal residual disease in bone marrow in advanced neuroblastoma: A pilot study [J].

Fukuda, M ;

Miyajima, Y ;

Miyashita, Y ;

Horibe, K .

JOURNAL OF PEDIATRIC HEMATOLOGY ONCOLOGY, 2001, 23 (01) :10-13

← 1 2 3 4 5 6 →