Anaplasma phagocytophilum Inhibits Apoptosis and Promotes Cytoskeleton Rearrangement for Infection of Tick Cells

被引：61

作者：

Ayllon, Nieves ^{[1
]}

Villar, Margarita ^{[1
]}

Busby, Ann T. ^{[2
]}

Kocan, Katherine M. ^{[2
]}

Blouin, Edmour F. ^{[2
]}

Bonzon-Kulichenko, Elena ^{[3
]}

Galindo, Ruth C. ^{[1
,2
]}

Mangold, Atilio J. ^{[4
]}

Alberdi, Pilar ^{[1
]}

Perez de la Lastra, Jose M. ^{[1
]}

Vazquez, Jesus ^{[3
]}

de la Fuente, Jose ^{[1
,2
]}

机构：

[1] Inst Invest Recursos Cineget IREC CSIC UCLM JCCM, Ciudad Real, Spain

[2] Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 USA

[3] Ctr Biol Mol Severo Ochoa CSIC UAM, Madrid, Spain

[4] Inst Nacl Tecnol Agr, Estn Expt Agr Rafaela, Santa Fe, Argentina

来源：

INFECTION AND IMMUNITY | 2013年 / 81卷 / 07期

关键词：

PROTECTIVE ANTIGEN SUBOLESIN; GENE-EXPRESSION; VECTOR INFESTATIONS; A-PHAGOCYTOPHILUM; MARGINALE; EVOLUTION; PROTEINS; PHOSPHORYLATION; MODULATION; SURVIVAL;

D O I：

10.1128/IAI.00194-13

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Anaplasma phagocytophilum causes human granulocytic anaplasmosis. Infection with this zoonotic pathogen affects gene expression in both the vertebrate host and the tick vector, Ixodes scapularis. Here, we identified new genes, including spectrin alpha chain or alpha-fodrin (CG8) and voltage-dependent anion-selective channel or mitochondrial porin (T2), that are involved in A. phagocytophilum infection/multiplication and the tick cell response to infection. The pathogen downregulated the expression of CG8 in tick salivary glands and T2 in both the gut and salivary glands to inhibit apoptosis as a mechanism to subvert host cell defenses and increase infection. In the gut, the tick response to infection through CG8 upregulation was used by the pathogen to increase infection due to the cytoskeleton rearrangement that is required for pathogen infection. These results increase our understanding of the role of tick genes during A. phagocytophilum infection and multiplication and demonstrate that the pathogen uses similar strategies to establish infection in both vertebrate and invertebrate hosts.

引用

页码：2415 / 2425

页数：11

共 64 条

[1] Identification of protective antigens for the control of Ixodes scapularis infestations using cDNA expression library immunization [J].

Almazán, C ;

Kocan, KM ;

Bergman, DK ;

Garcia-Garcia, JC ;

Blouin, EF ;

de la Fuente, J .

VACCINE, 2003, 21 (13-14) :1492-1501

[2] Exploring the mialome of ticks: an annotated catalogue of midgut transcripts from the hard tick, Dermacentor variabilis (Acari: Ixodidae) [J].

Anderson, Jennifer M. ;

Sonenshine, Daniel E. ;

Valenzuela, Jesus G. .

BMC GENOMICS, 2008, 9 (1)

[3] Structure of the human voltage-dependent anion channel [J].

Bayrhuber, Monika ;

Meins, Thomas ;

Habeck, Michael ;

Becker, Stefan ;

Giller, Karin ;

Villinger, Saskia ;

Vonrhein, Clemens ;

Griesinger, Christian ;

Zweckstetter, Markus ;

Zeth, Kornelius .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (40) :15370-15375

[4] Expression of heat shock proteins and subolesin affects stress responses, Anaplasma phagocytophilum infection and questing behaviour in the tick, Ixodes scapularis [J].

Busby, A. T. ;

Ayllon, N. ;

Kocan, K. M. ;

Blouin, E. F. ;

De La Fuente, G. ;

Galindo, R. C. ;

Villar, M. ;

De La Fuente, J. .

MEDICAL AND VETERINARY ENTOMOLOGY, 2012, 26 (01) :92-102

[5]

Colombini M, 1996, Ion Channels, V4, P169

[6] Gene expression profiling of human promyelocytic cells in response to infection with Anaplasma phagocytophilum [J].

de la Fuente, J ;

Ayoubi, P ;

Blouin, EF ;

Almazán, C ;

Naranjo, V ;

Kocan, KM .

CELLULAR MICROBIOLOGY, 2005, 7 (04) :549-559

[7] Overview: Ticks as vectors of pathogens that cause disease in humans and animals [J].

de la Fuente, Jose ;

Estrada-Pena, Agustin ;

Venzal, Jose M. ;

Kocan, Katherine M. ;

Sonenshine, Daniel E. .

FRONTIERS IN BIOSCIENCE-LANDMARK, 2008, 13 :6938-6946

[8] Reduction of tick infections with Anaplasma marginale and A-phagocytophilum by targeting the tick protective antigen subolesin [J].

de la Fuente, Jose ;

Almazan, Consuelo ;

Blouin, Edmour F. ;

Naranjo, Victoria ;

Kocan, Katherine M. .

PARASITOLOGY RESEARCH, 2006, 100 (01) :85-91

[9] The tick protective antigen, 4D8, is a conserved protein involved in modulation of tick blood ingestion and reproduction [J].

de la Fuente, Jose ;

Almazan, Consuelo ;

Blas-Machado, Uriel ;

Naranjo, Victoria ;

Mangold, Atilio J. ;

Blouin, Edmour F. ;

Gortazar, Christian ;

Kocan, Katherine M. .

VACCINE, 2006, 24 (19) :4082-4095

[10] Synergistic effect of silencing the expression of tick protective antigens 4D8 and Rs86 in Rhipicephalus sanguineus by RNA interference [J].

de la Fuente, José ;

Almazán, Consuelo ;

Naranjo, Victoria ;

Blouin, Edmour F. ;

Kocan, Katherine M. .

PARASITOLOGY RESEARCH, 2006, 99 (02) :108-113

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