GENETIC DIVERSITY OF ACUTE BEE PARALYSIS VIRUS IN SLOVENIAN HONEYBEE SAMPLES

被引：9
作者：
Ciglenecki, Urska Jamnikar [1 ]
Toplak, Ivan [1 ]
机构：
[1] Univ Ljubljana, Fac Vet, Inst Microbiol & Parasitol, SI-1115 Ljubljana, Slovenia
来源：
ACTA VETERINARIA HUNGARICA | 2013年 / 61卷 / 02期
关键词：
Honeybee; acute bee paralysis virus; ORF 1 and ORF 2; phylogenetic analysis; APIS-MELLIFERA L; DEFORMED WING VIRUS; VARROA-DESTRUCTOR; INFECTIONS; POPULATIONS; PREVALENCE; COLONIES; KASHMIR; PCR;
D O I：
10.1556/AVet.2013.005
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The genetic diversity of acute bee paralysis virus (ABPV) in honeybees was studied in Slovenia. A total of 248 honeybee samples obtained from 134 different apiaries in Slovenia were tested for the presence of ABPV by RT-PCR. Specific 398-base pair (bp) products were generated with primers amplifying the ORF2 region and 452-base pair (bp) products with primers amplifying the ORF1 region of the viral genome. To characterise the overall nucleotide diversity among the ABPV sequences, phylogenetic trees with 54 and 29 samples were constructed from 357 nucleotides from ORF2 and 408 nucleotides from ORF1, respectively. The nucleotide comparison of Slovenian ABPV strains revealed two distinct clusters in ORF2 and ORF1, showing 91.2-92.5% and 96.7-97.2% nucleotide identity, respectively. Comparison of data regarding the geographical location of the ABPV-positive samples with the constructed phylogenetic trees revealed the random distribution of the two clusters throughout Slovenia.
引用
收藏
页码：244 / 256
页数：13
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