mRNA up-regulation of MHC II and pivotal pro-inflammatory genes in normal brain aging

被引：251
作者：
Frank, MG
Barrientos, RA
Biedenkapp, JC
Rudy, JW
Watkins, LR
Maier, SF
机构：
[1] Univ Colorado, Dept Psychol, Boulder, CO 80309 USA
[2] Univ Colorado, Ctr Neurosci, Boulder, CO 80309 USA
来源：
NEUROBIOLOGY OF AGING | 2006年 / 27卷 / 05期
关键词：
aging; macrophage; microglia; immune; pro-inflammatory; MHC II; hippocampus;
D O I：
10.1016/j.neurobiolaging.2005.03.013
中图分类号：
R592 [老年病学]; C [社会科学总论];
学科分类号：
03 ; 0303 ; 100203 ;
摘要：
In normal brain aging, CNS resident macrophages exhibit increased expression of major histocompatibility complex (MHC) 11 expression. However, the transcriptional basis for this observation has not been clarified nor have age-related alterations in pivotal pro-inflammatory genes been characterized. Age-related mRNA alterations in MHC 11, MHC 11 accessory molecules and several pro-inflammatory mediators were measured in older (24 months) and younger (3 months) male F344xBN F1 rats. Real time RT-PCR was utilized to measure steady state mRNA levels in hippocarnpus. Older as compared to younger animals exhibited increased mRNA levels of MHC II, CD86, CIITA and IFN-gamma. Furthermore, IL-10 and CD200 mRNA, molecules that down-regulate macrophage activation, was decreased in older animals. The present results indicate that normal brain aging is characterized by a shift towards a pro-inflammatory microenvironment in the CNS. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码：717 / 722
页数：6
相关论文
共 53 条
[1]   Immune function of microglia [J].
Aloisi, F .
GLIA, 2001, 36 (02) :165-179
[2]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[3]   CD200 and membrane protein E interactions in the control of myeloid cells [J].
Barclay, AN ;
Wright, GJ ;
Brooke, G ;
Brown, MH .
TRENDS IN IMMUNOLOGY, 2002, 23 (06) :285-290
[4]  
Blalock EM, 2003, J NEUROSCI, V23, P3807
[5]   Transcriptional regulation of the MHC class II antigen presentation pathway [J].
Boss, JM ;
Jensen, PE .
CURRENT OPINION IN IMMUNOLOGY, 2003, 15 (01) :105-111
[6]   Comparison of gene expression in old versus young rat hippocampus by cDNA array [J].
Cho, KS ;
Choi, JG ;
Ha, CM ;
Son, YJ ;
Choi, WS ;
Lee, BJ .
NEUROREPORT, 2002, 13 (03) :285-289
[7]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[8]   Immune function of astrocytes [J].
Dong, YS ;
Benveniste, EN .
GLIA, 2001, 36 (02) :180-190
[9]   Neurons, glia, and plasticity in normal brain aging [J].
Finch, CE .
NEUROBIOLOGY OF AGING, 2003, 24 :S123-S127
[10]   Discussion to 'Imaging methods for evaluating brain function in man' by Robert M. Kessler [J].
Finch, CE ;
Kessler, R ;
Brosens, I ;
Smith, J ;
Saletu, B ;
Goodwin, GM ;
Buitelaar, JK .
NEUROBIOLOGY OF AGING, 2003, 24 :S37-S39
123456