A novel AIEgen-based probe for detecting cysteine in lipid droplets

被引：34

作者：

Cheng, Wei ^{[1
]}

Xue, Xuqi ^{[1
]}

Zhang, Fang ^{[1
]}

Zhang, Baoxin ^{[1
]}

Li, Taihan ^{[3
]}

Peng, Liang ^{[2
]}

Cho, Dong-Hyung ^{[4
]}

Chen, Hongli ^{[1
]}

Fang, Jianguo ^{[1
]}

Chen, Xingguo ^{[1
]}

机构：

[1] Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China

[2] Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China

[3] Lanzhou Univ, Coll Basic Med Sci, Lanzhou 730000, Peoples R China

[4] Kyungpook Natl Univ, Sch Life Sci, Daegu 41566, South Korea

来源：

ANALYTICA CHIMICA ACTA | 2020年 / 1127卷

基金：

中国国家自然科学基金;

关键词：

Aggregation-induced emission; Cysteine detection; Cell imaging; Fluorescent probe; Lipid droplets; HIGHLY SELECTIVE DETECTION; OFF-ON PROBE; FLUORESCENT-PROBE; GLUTATHIONE; HOMOCYSTEINE; CONJUGATE; EMISSION; SENSOR; CYS; GSH;

D O I：

10.1016/j.aca.2020.05.074

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A smart fluorescent probe DPAS-Cys has been rationally designed based on a typical AIEgen DPAS and an acrylate moiety. The probe DPAS-Cys not only can be used for the detection of cysteine (Cys) selectively with large Stokes shift (200 nm) and relatively low detection limit (2.4 mu M), but also shows lipid droplets (LDs) targeting property. The response mechanism for Cys was carefully verified. Importantly, due to the aggregation-induced emission characteristic, the introduction of considerable percentage of traditional organic solvent is avoidable, which makes it suitable for bioimaging in physiological systems. In addition, the confocal fluorescence imaging demonstrates that DPAS-Cys is able to detect Cys in LDs of different cell lines with universality. Our study opens a new avenue to understand the importance of LDs in biosystem, for which the gap between the essential biothiol Cys and the energy storage organelle LDs was bridged for the first time. (C) 2020 Elsevier B.V. All rights reserved.

引用

页码：20 / 28

页数：9

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