An intermediate seeks instant gratification

被引：11

作者：

Gruebele, M

机构：

[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Phys, Urbana, IL 61801 USA

[3] Univ Illinois, Ctr Biophys & Computat Biol, Urbana, IL 61801 USA

来源：

NATURE STRUCTURAL BIOLOGY | 2002年 / 9卷 / 03期

关键词：

D O I：

10.1038/nsb0302-154

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phi-value analysis of the four-helix protein Im7 reveals that a folding intermediate with non-native structure rapidly forms en route to the native state, even though the protein topology is very simple. Energetic frustration, at least partially induced by functional constraints, appears to play a significant role.

引用

收藏

页码：154 / 155

页数：2

相关论文

共 10 条

[1]

Capaldi AP, 2001, NAT STRUCT BIOL, V8, P68

[2] Im7 folding mechanism: misfolding on a path to the native state [J].

Capaldi, AP ;

Kleanthous, C ;

Radford, SE .

NATURE STRUCTURAL BIOLOGY, 2002, 9 (03) :209-216

[3] Topological and energetic factors: What determines the structural details of the transition state ensemble and "en-route" intermediates for protein folding? An investigation for small globular proteins [J].

Clementi, C ;

Nymeyer, H ;

Onuchic, JN .

JOURNAL OF MOLECULAR BIOLOGY, 2000, 298 (05) :937-953

[4] THE FOLDING OF AN ENZYME .1. THEORY OF PROTEIN ENGINEERING ANALYSIS OF STABILITY AND PATHWAY OF PROTEIN FOLDING [J].

FERSHT, AR ;

MATOUSCHEK, A ;

SERRANO, L .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 224 (03) :771-782

[5] The folding mechanism of a β-sheet:: The WW domain [J].

Jäger, M ;

Nguyen, H ;

Crane, JC ;

Kelly, JW ;

Gruebele, M .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 311 (02) :373-393

[6] A simple model for calculating the kinetics of protein folding from three-dimensional structures [J].

Muñoz, V ;

Eaton, WA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (20) :11311-11316

[7] Alternative explanations for "multistate" kinetics in protein folding: Transient aggregation and changing transition-state ensembles [J].

Oliveberg, M .

ACCOUNTS OF CHEMICAL RESEARCH, 1998, 31 (11) :765-772

[8] Energetic frustration and the nature of the transition state in protein folding [J].

Shea, JE ;

Onuchic, JN ;

Brooks, CL .

JOURNAL OF CHEMICAL PHYSICS, 2000, 113 (17) :7663-7671

[9] Intermediates can accelerate protein folding [J].

Wagner, C ;

Kiefhaber, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (12) :6716-6721

[10] NAVIGATING THE FOLDING ROUTES [J].

WOLYNES, PG ;

ONUCHIC, JN ;

THIRUMALAI, D .

SCIENCE, 1995, 267 (5204) :1619-1620