Establishment of Cellobiose Utilization for Lipid Production in Rhodococcus opacus PD630

被引:31
作者
Hetzler, Stephan [1 ]
Steinbuechel, Alexander [1 ,2 ]
机构
[1] Univ Munich, Inst Mol Mikrobiol & Biotechnol, Munster, Germany
[2] King Abdulaziz Univ, Dept Environm Sci, Jeddah 21413, Saudi Arabia
关键词
BIOFUELS; TRIACYLGLYCEROLS; FERMENTATION; BIOLOGY;
D O I
10.1128/AEM.03678-12
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Rhodococcus opacus PD630, which is known for its ability to accumulate large amounts of triacylglycerols (TAG), was metabolically engineered, and a cellobiose utilization pathway was introduced. Activities of beta-glucosidases were determined, and recombinant strains accumulated fatty acids up to 39.5 +/- 5.7% (wt/wt) of cell dry mass from cellobiose.
引用
收藏
页码:3122 / 3125
页数:4
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