A Role for the ESCRT System in Cell Division in Archaea

被引:259
作者
Samson, Rachel Y. [1 ]
Obita, Takayuki [2 ]
Freund, Stefan M. [3 ]
Williams, Roger L. [2 ]
Bell, Stephen D. [1 ]
机构
[1] MRC, Canc Cell Unit, Cambridge CB2 0XZ, England
[2] MRC, Mol Biol Lab, Cambridge CB2 0QH, England
[3] MRC, Ctr Prot Engn, Cambridge CB2 0QH, England
基金
英国惠康基金; 英国医学研究理事会;
关键词
D O I
10.1126/science.1165322
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Archaea are prokaryotic organisms that lack endomembrane structures. However, a number of hyperthermophilic members of the Kingdom Crenarchaea, including members of the Sulfolobus genus, encode homologs of the eukaryotic endosomal sorting system components Vps4 and ESCRT- III ( endosomal sorting complex required for transport- III). We found that Sulfolobus ESCRT- III and Vps4 homologs underwent regulation of their expression during the cell cycle. The proteins interacted and we established the structural basis of this interaction. Furthermore, these proteins specifically localized to the mid- cell during cell division. Overexpression of a catalytically inactive mutant Vps4 in Sulfolobus resulted in the accumulation of enlarged cells, indicative of failed cell division. Thus, the archaeal ESCRT system plays a key role in cell division.
引用
收藏
页码:1710 / 1713
页数:4
相关论文
共 21 条
[1]   A dynamic bacterial cytoskeleton [J].
Carballido-Löpez, R ;
Errington, J .
TRENDS IN CELL BIOLOGY, 2003, 13 (11) :577-583
[2]   Parallels between cytokinesis and retroviral budding: A role for the ESCRT machinery [J].
Carlton, Jez G. ;
Martin-Serrano, Juan .
SCIENCE, 2007, 316 (5833) :1908-1912
[3]   Chromosome replication dynamics in the archaeon Sulfolobus acidocaldarius [J].
Duggin, Iain G. ;
McCallum, Simon A. ;
Bell, Stephen D. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (43) :16737-16742
[4]   The push and pull of the bacterial cytoskeleton [J].
Dye, Natalie A. ;
Shapiro, Lucy .
TRENDS IN CELL BIOLOGY, 2007, 17 (05) :239-245
[5]   Beyond Tsg101: the role of Alix in 'ESCRTing' HIV-1 [J].
Fujii, Ken ;
Hurley, James H. ;
Freed, Eric O. .
NATURE REVIEWS MICROBIOLOGY, 2007, 5 (12) :912-916
[6]   The Sulfolobus solfataricus AAA protein sso090, a homologue of the eukaryotic ESCRT vps4 ATPase [J].
Hobel, Cedric F. V. ;
Albers, Sonja V. ;
Driessen, Arnold J. M. ;
Lupas, Andrei N. .
BIOCHEMICAL SOCIETY TRANSACTIONS, 2008, 36 :94-98
[7]   The ESCRT complexes: Structure and mechanism of a membrane-trafficking network [J].
Hurley, James H. ;
Emr, Scott D. .
ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 2006, 35 :277-298
[8]   Archaeal dominance in the mesopelagic zone of the Pacific Ocean [J].
Karner, MB ;
DeLong, EF ;
Karl, DM .
NATURE, 2001, 409 (6819) :507-510
[9]   Two distinct modes of ESCRT-III recognition are required for VPS4 functions in lysosomal protein targeting and HIV-1 budding [J].
Kieffer, Collin ;
Skalicky, Jack J. ;
Morita, Eiji ;
De Domenico, Ivana ;
Ward, Diane M. ;
Kaplan, Jerry ;
Sundquist, Wesley I. .
DEVELOPMENTAL CELL, 2008, 15 (01) :62-73
[10]   Regulation of expression of the arabinose and glucose transporter genes in the thermophilic archaeon Sulfolobus solfataricus [J].
Lubelska, Joanna M. ;
Jonuscheit, Melanie ;
Schleper, Christa ;
Albers, Sonja-Verena ;
Driessen, Arnold J. M. .
EXTREMOPHILES, 2006, 10 (05) :383-391