Development and validation of a method for detection and quantification of ochratoxin A in green coffee using liquid chromatography coupled to mass spectrometry

被引：3

作者：

da Costa Cunha Bandeira, Raquel Duarte ^{[1
]}

Uekane, Thais Matsue ^{[1
]}

da Cunha, Carolina Passos ^{[1
]}

da Cunha, Valnei Smarcaro ^{[1
]}

Rodrigues, Janaina Marques ^{[1
]}

de Oliveira Godoy, Ronoel Luiz ^{[2
]}

Campino de la Cruz, Marcus Henrique ^{[3
]}

机构：

[1] Standardizat & Ind Qual INMETRO, Natl Inst Metrol, Organ Anal Lab LABOR, Sci & Ind Metrol Div DIMCI,Chem Div Metrol, BR-25250020 Duque De Caxias, RJ, Brazil

[2] Minist Agr, Livestock & Food Supply EMBRAPA, Brazilian Agr Res Corp, Embrapa Food Technol, Rio De Janeiro, RJ, Brazil

[3] Oswaldo Cruz Fdn FIOCRUZ, Natl Inst Qual Control Hlth INCQS, Rio De Janeiro, RJ, Brazil

来源：

CIENCIA E TECNOLOGIA DE ALIMENTOS | 2012年 / 32卷 / 04期

关键词：

coffee; mycotoxin; validation; HPLC; MS/MS; IMMUNOAFFINITY COLUMN CLEANUP; SOLID-PHASE CLEANUP; ROASTED COFFEE; BEANS; HPLC;

D O I：

10.1590/S0101-20612012005000120

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A method using Liquid Chromatography Tanden Mass Spectrometry (LC-MS/MS) with matrix-matched calibration curve was developed and validated for determining ochratoxin A (OTA) in green coffee. Linearity was found between 3.0 and 23.0 ng.g(-1). Mean recoveries ranged between 90.45% and 108.81%; the relative standard deviation under repeatability and intermediate precision conditions ranged from 5.39% to 9.94% and from 2.20% to 14.34%, respectively. The limits of detection and quantification were 1.2 ng.g(-1) and 3.0 ng.g(-1), respectively. The method developed was suitable and contributed to the field of mycotoxin analysis, and it will be used for future production of the Certified Reference Material (CRM) for OTA in coffee.

引用

页码：775 / 782

页数：8

共 40 条

[1] Evaluation of methods used to determine ochratoxin a in coffee beans
Ahmed, N. E.
Farag, M. M.
Soliman, K. M.
Abdel-Samed, A. K. M.
Naguib, Kh. M.
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (23) : 9576 - 9580
[2] ASSOCIATION OF ANALYTICAL COMMUNITIES-AOAC INTERNATIONAL, 2005, OFF METH AN AOAC INT, P58
[3] Column liquid chromatography electrospray ionisation tandem mass spectrometry for the analysis of ochratoxin
Becker, M
Degelmann, P
Herderich, M
Schreier, P
Humpf, HU
[J]. JOURNAL OF CHROMATOGRAPHY A, 1998, 818 (02) : 260 - 264
[4] Bresch H, 2000, ARCH LEBENSMITTELHYG, V51, P89
[5] Research on the origin, and on the impact of post-harvest handling and manufacturing on the presence of ochratoxin A in coffee
Bucheli, P
Taniwaki, MH
[J]. FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2002, 19 (07): : 655 - 665
[6] Bullerman L.B., 2003, ENCY FOOD SCI NUTR, P4080, DOI DOI 10.1016/130-12-227055-X/00821-X
[7] CAC (Codex Alimentarius Commission), 2015, PROC MAN
[8] Ensuring both normality and homocedasticity of chromatographic data-ratios for internal-standard least-squares calibration
Cuadros-Rodriguez, L
Gonzalez-Casado, A
Garcia-Campana, AM
Vilchez, JL
[J]. CHROMATOGRAPHIA, 1998, 47 (9-10) : 550 - 556
[9] Ochratoxin A in Brazilian instant coffee
de Almeida, Adriana P.
Alaburda, Janete
Shundo, Luzia
Ruvieri, Valter
Navas, Sandra A.
Lamardo, Leda C. A.
Sabino, Myrna
[J]. BRAZILIAN JOURNAL OF MICROBIOLOGY, 2007, 38 (02) : 300 - 303
[10] Method validation for the determination of ochratoxin A in green and soluble coffee by immunoaffinity column cleanup and liquid chromatography
Diaz G.J.
Ariza D.
Perula N.S.
[J]. Mycotoxin Research, 2004, 20 (2) : 59 - 67

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