Integrin Beta 1 Suppresses Multilayering of a Simple Epithelium

被引:16
作者
Chen, Jichao
Krasnow, Mark A.
机构
[1] Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA
[2] Stanford Univ, Sch Med, HHMI, Stanford, CA 94305 USA
来源
PLOS ONE | 2012年 / 7卷 / 12期
关键词
LUNG BRANCHING MORPHOGENESIS; EPIDERMAL MORPHOGENESIS; EXPRESSION; SKIN; BETA-1-INTEGRIN; STRATIFICATION; MOUSE; P63; DIFFERENTIATION; PROLIFERATION;
D O I
10.1371/journal.pone.0052886
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Epithelia are classified as either simple, a single cell layer thick, or stratified (multilayered). Stratified epithelia arise from simple epithelia during development, and transcription factor p63 functions as a key positive regulator of epidermal stratification. Here we show that deletion of integrin beta 1 (Itgb1) in the developing mouse airway epithelium abrogates airway branching and converts this monolayer epithelium into a multilayer epithelium with more than 10 extra layers. Mutant lung epithelial cells change mitotic spindle orientation to seed outer layers, and cells in different layers become molecularly and functionally distinct, hallmarks of normal stratification. However, mutant lung epithelial cells do not activate p63 and do not switch to the stratified keratin profile of epidermal cells. These data, together with previous data implicating Itgb1 in regulation of epidermal stratification, suggest that the simple-versus-stratified developmental decision may involve not only stratification inducers like p63 but suppressors like Itgb1 that prevent simple epithelia from inappropriately activating key steps in the stratification program.
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页数:8
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