Simultaneous Saccharification and Ethanol Fermentation of Corn Stover at High Temperature and High Solids Loading by a Thermotolerant Strain Saccharomyces cerevisiae DQ1

被引：32
作者：
Chu, Deqiang [1 ]
Zhang, Jian [1 ]
Bao, Jie [1 ]
机构：
[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
来源：
BIOENERGY RESEARCH | 2012年 / 5卷 / 04期
基金：
中国博士后科学基金;
关键词：
Simultaneous saccharification and ethanol fermentation (SSF); Thermotolerant yeast; Saccharomyces cerevisiae DQ1; High solids loading; Corn stover; KLUYVEROMYCES-MARXIANUS; SSF; SELECTION; WASTES;
D O I：
10.1007/s12155-012-9219-x
中图分类号：
TE [石油、天然气工业]; TK [能源与动力工程];
学科分类号：
0807 ; 0820 ;
摘要：
The thermotolerant strain Saccharomyces cerevisiae DQ1 was applied to the simultaneous saccharification and fermentation (SSF) at high temperature and high solids loading of the dilute acid-pretreated corn stover in the present study. The SSF using S. cerevisiae DQ1 was operated at 30 % solids loading of the pretreated corn stover with three-step SSF mode and achieved up to ethanol titer of 48 g/L and yield of 65.6 %. S. cerevisiae DQ1 showed strong thermotolerance in both the regular one-step SSF and the three-step SSF with changing temperature in each step. The three-step SSF at 40A degrees C using S. cerevisiae DQ1 tolerated the greater cellulase dosage and solids loading of the pretreated corn stover and resulted in increased ethanol production. The present study provided a practical potential for the future SSF of lignocellulose feedstock at high temperature to reach high ethanol titer.
引用
收藏
页码：1020 / 1026
页数：7
相关论文
共 19 条
[1]   High-temperature fermentation: how can processes for ethanol production at high temperatures become superior to the traditional process using mesophilic yeast? [J].
Abdel-Banat, Babiker M. A. ;
Hoshida, Hisashi ;
Ano, Akihiko ;
Nonklang, Sanom ;
Akada, Rinji .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2010, 85 (04) :861-867
[2]   Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae [J].
Almeida, Jodo R. M. ;
Modig, Tobias ;
Petersson, Anneli ;
Hahn-Hagerdal, Barbel ;
Liden, Gunnar ;
Gorwa-Grauslund, Marie F. .
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2007, 82 (04) :340-349
[3]   Utilization of dry distiller's grain and solubles as nutrient supplement in the simultaneous saccharification and ethanol fermentation at high solids loading of corn stover [J].
Bi, Dexi ;
Chu, Deqiang ;
Zhu, Pei ;
Lu, Chenyang ;
Fan, Chao ;
Zhang, Jian ;
Bao, Jie .
BIOTECHNOLOGY LETTERS, 2011, 33 (02) :273-276
[4]  
Edgardo A, 2008, ENZYME MICROB TECH, V43, P120, DOI [10.1016/j.enzmictec.2008.02.007, 10.1016/j.enzmictec.2007.08.006]
[5]   Ethanol production through simultaneous saccharification and fermentation of switchgrass using Saccharomyces cerevisiae D5A and thermotolerant Kluyveromyces marxianus IMB strains [J].
Faga, Brian A. ;
Wilkins, Mark R. ;
Banat, Ibrahim M. .
BIORESOURCE TECHNOLOGY, 2010, 101 (07) :2273-2279
[6]   Pretreatment of lignocellulosic materials for efficient bioethanol production [J].
Galbe, Mats ;
Zacchi, Guido .
BIOFUELS, 2007, 108 :41-65
[7]   Characteristics of cellulase preparations affecting the simultaneous saccharification and fermentation of cellulose to ethanol [J].
Golias, H ;
Dumsday, GJ ;
Stanley, GA ;
Pamment, NB .
BIOTECHNOLOGY LETTERS, 2000, 22 (07) :617-621
[8]   Inhibition Performance of Lignocellulose Degradation Products on Industrial Cellulase Enzymes During Cellulose Hydrolysis [J].
Jing, Xinyun ;
Zhang, Xiaoxi ;
Bao, Jie .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2009, 159 (03) :696-707
[9]   Effect of Nutrients on Fermentation of Pretreated Wheat Straw at very High Dry Matter Content by Saccharomyces cerevisiae [J].
Jorgensen, Henning .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2009, 153 (1-2) :44-57
[10]   Simultaneous saccharification and fermentation (SSF) of industrial wastes for the production of ethanol [J].
Kádár, Z ;
Szengyel, Z ;
Réczey, K .
INDUSTRIAL CROPS AND PRODUCTS, 2004, 20 (01) :103-110
12