Faster than the Speed of Hearing: Nanomechanical Force Probes Enable the Electromechanical Observation of Cochlear Hair Cells

被引：30

作者：

Doll, Joseph C. ^{[1
]}

Peng, Anthony W. ^{[2
]}

Ricci, Anthony J. ^{[2
]}

Pruitt, Beth L. ^{[1
]}

机构：

[1] Stanford Univ, Dept Mech Engn, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Otolaryngol Head & Neck Surg, Stanford, CA 94305 USA

来源：

NANO LETTERS | 2012年 / 12卷 / 12期

关键词：

NEMS; MEMS; piezoresistive; piezoelectric; hair cell; hearing; OPTICAL TWEEZERS; CANTILEVERS; TRANSDUCTION; MICROSCOPY; MEMBRANE; BUNDLES;

D O I：

10.1021/nl3036349

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Understanding the mechanisms responsible for our sense of hearing requires new tools for unprecedented stimulation and monitoring of sensory cell mechanotransduction at frequencies yet to be explored. We describe nanomechanical force probes designed to evoke mechanotransduction currents at up to 100 kHz in living cells. High-speed force and displacement metrology is enabled by integrating piezoresistive sensors and piezoelectric actuators onto nanoscale cantilevers. The design, fabrication process, actuator performance, and actuator-sensor crosstalk compensation results are presented. We demonstrate the measurement of mammalian cochlear hair cell mechanotransduction with simultaneous patch clamp recordings at unprecedented speeds. The probes can deliver mechanical stimuli with sub-10 mu s rise times in water and are compatible with standard upright and inverted microscopes.

引用

页码：6107 / 6111

页数：5

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