Solution-state 2D NMR of Ball-milled Plant Cell Wall Gels in DMSO-d6

被引：252

作者：

Kim, Hoon ^{[1
,2
]}

Ralph, John ^{[1
,3
,4
]}

Akiyama, Takuya ^{[1
]}

机构：

[1] ARS, US Dairy Forage Res Ctr, USDA, Madison, WI 53706 USA

[2] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA

[3] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA

[4] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA

来源：

BIOENERGY RESEARCH | 2008年 / 1卷 / 01期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

Plant cell wall; Biomass; Lignin; Polysaccharide; Gel-state; 2DNMR; HMQC; HSQC; DMSO; Pine; Aspen; Kenaf; Corn;

D O I：

10.1007/s12155-008-9004-z

中图分类号：

TE [石油、天然气工业]; TK [能源与动力工程];

学科分类号：

0807 ; 0820 ;

摘要：

Although finely divided ball-milled whole cell walls do not completely dissolve in dimethylsulfoxide (DMSO), they readily swell producing a gel. Solution-state two-dimensional (2D) nuclear magnetic resonance (NMR) of this gel, produced directly in the NMR tube, provides an interpretable structural fingerprint of the polysaccharide and lignin components of the wall without actual solubilization, and without structural modification beyond that inflicted by the ball milling and ultrasonication steps. Since the cellulose is highly crystalline and difficult to swell, the componentmay be under-represented in the spectra. The method however provides a more rapid method for comparative structural evaluation of plant cell walls than is currently available. With the new potential for chemometric analysis using the 2D NMR fingerprint, this method may find application as a secondary screen for selecting biomass lines and for optimizing biomass processing and conversion efficiencies.

引用

页码：56 / 66

页数：11

共 48 条

[1] Studies on the homogeneous acetylation of cellulose in the novel solvent dimethyl sulfoxide/tetrabutylammonium fluoride trihydrate [J].

Ass, BAP ;

Frollini, E ;

Heinze, T .

MACROMOLECULAR BIOSCIENCE, 2004, 4 (11) :1008-1013

[2] 13C assignments of the carbon atoms in the aromatic rings of lignin model compounds of the arylglycerol β-aryl ether type [J].

Bardet, Michel ;

Lundquist, Knut ;

Parkas, Jim ;

Robert, Danielle ;

von Unge, Sverker .

MAGNETIC RESONANCE IN CHEMISTRY, 2006, 44 (10) :976-979

[3] ISOLATION OF LIGNIN FROM FINELY DIVIDED WOOD WITH NEUTRAL SOLVENTS [J].

BJORKMAN, A .

NATURE, 1954, 174 (4440) :1057-1058

[4] Lignin biosynthesis [J].

Boerjan, W ;

Ralph, J ;

Baucher, M .

ANNUAL REVIEW OF PLANT BIOLOGY, 2003, 54 :519-546

[5] Quantitative characterization of a hardwood milled wood lignin by nuclear magnetic resonance spectroscopy [J].

Capanema, EA ;

Balakshin, MY ;

Kadla, JF .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (25) :9639-9649

[6] H-1-NMR AND C-13-NMR CHARACTERIZATION OF THE DIGALACTOSYLMANNOPENTAOSE LIBERATED FROM LEGUME SEED GALACTOMANNAN BY BETA-MANNANASE ACTION [J].

DAVIS, AL ;

HOFFMANN, RA ;

RUSSELL, AL ;

DEBET, M .

CARBOHYDRATE RESEARCH, 1995, 271 (01) :43-54

[7] Identifying acetylated lignin units in non-wood fibers using pyrolysis-gas chromatography/mass spectrometry [J].

del Río, JC ;

Gutiérrez, A ;

Martínez, AT .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2004, 18 (11) :1181-1185

[8] Occurrence of naturally acetylated lignin units [J].

Del Rio, Jose C. ;

Marques, Gisela ;

Rencoret, Jorge ;

Martinez, Angel T. ;

Gutierrez, Ana .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (14) :5461-5468

[9]

Fengel D., 1989, WOOD CHEM ULTRASTRUC

[10]

Fort DA, 2007, GREEN CHEM, V9, P63, DOI 10.1039/B607614A

← 1 2 3 4 5 →