Alignment-free, all-spliced fiber laser source for CARS microscopy based on four-wave-mixing

被引：64

作者：

Baumgart, Martin ^{[1
]}

Gottschall, Thomas ^{[1
]}

Abreu-Afonso, Javier ^{[2
]}

Diez, Antonio ^{[2
]}

Meyer, Tobias ^{[3
,4
]}

Dietzek, Benjamin ^{[3
,4
]}

Rothhardt, Manfred ^{[4
]}

Popp, Juergen ^{[3
,4
]}

Limpert, Jens ^{[1
]}

Tuennermann, Andreas ^{[1
,5
]}

机构：

[1] Univ Jena, Inst Appl Phys, Abbe Ctr Photon, D-07745 Jena, Germany

[2] Univ Valencia, Dept Fis Aplicada ICMUV, E-46100 Burjassot, Spain

[3] Univ Jena, Inst Phys Chem, Abbe Ctr Photon, D-07743 Jena, Germany

[4] Inst Photon Technol IPHT eV, D-07745 Jena, Germany

[5] Fraunhofer Inst Appl Opt & Precis Engn, D-07745 Jena, Germany

来源：

OPTICS EXPRESS | 2012年 / 20卷 / 19期

关键词：

RAMAN SCATTERING MICROSCOPY; GENERATION; SYSTEM; LIGHT; PULSE;

D O I：

10.1364/OE.20.021010

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

An environmentally-stable low-repetition rate fiber oscillator is developed to produce narrow-bandwidth pulses with several tens of picoseconds duration. Based on this oscillator an alignment-free all-fiber laser for multi-photon microscopy is realized using in-fiber frequency conversion based on four-wave-mixing. Both pump and Stokes pulses for coherent anti-Stokes Raman scattering (CARS) microscopy are readily available from one fiber end, intrinsically overlapped in space and time, which drastically simplifies the experimental handling for the user. The complete laser setup is mounted on a home-built laser scanning microscope with small footprint. High-quality multimodal microscope images of biological tissue are presented probing the CH-stretching resonance of lipids at an anti-Stokes Raman-shift of 2845 cm(-1) and second-harmonic generation of collagen. Due to its simplicity, compactness, maintenance-free operation, and ease-of-use the presented low-cost laser is an ideal source for bio-medical applications outside laser laboratories and in particular inside clinics. (C) 2012 Optical Society of America

引用

页码：21010 / 21018

页数：9

共 18 条

[1] Fiber laser-based light source for coherent anti-Stokes Raman scattering microspectroscopy [J].

Andresen, Esben Ravn ;

Nielsen, Carsten Krogh ;

Thogersen, Jan ;

Keiding, Soren Rud .

OPTICS EXPRESS, 2007, 15 (08) :4848-4856

[2] Fiber delivered probe for efficient CARS imaging of tissues [J].

Balu, Mihaela ;

Liu, Gangjun ;

Chen, Zhongping ;

Tromberg, Bruce J. ;

Potma, Eric O. .

OPTICS EXPRESS, 2010, 18 (03) :2380-2388

[3] Impact of dispersion on pulse dynamics in chirped-pulse fiber lasers [J].

Baumgartl, M. ;

Ortac, B. ;

Limpert, J. ;

Tuennermann, A. .

APPLIED PHYSICS B-LASERS AND OPTICS, 2012, 107 (02) :263-274

[4] All-fiber laser source for CARS microscopy based on fiber optical parametric frequency conversion [J].

Baumgartl, Martin ;

Chemnitz, Mario ;

Jauregui, Cesar ;

Meyer, Tobias ;

Dietzek, Benjamin ;

Popp, Juergen ;

Limpert, Jens ;

Tuennermann, Andreas .

OPTICS EXPRESS, 2012, 20 (04) :4484-4493

[5] Coherent anti-Stokes Raman scattering hyperspectral tissue imaging with a wavelength-swept system [J].

Begin, Steve ;

Burgoyne, Bryan ;

Mercier, Vincent ;

Villeneuve, Alain ;

Vallee, Real ;

Cote, Daniel .

BIOMEDICAL OPTICS EXPRESS, 2011, 2 (05) :1296-1306

[6] Coherent Anti-Stokes Raman Scattering Microscopy: Chemical Imaging for Biology and Medicine [J].

Evans, Conor. L. ;

Xie, X. Sunney .

ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, 2008, 1 (01) :883-909

[7] Pulse-length dependence of cellular response to intense near-infrared laser pulses in multiphoton microscopes [J].

König, K ;

Becker, TW ;

Fischer, P ;

Riemann, I ;

Halbhuber, KJ .

OPTICS LETTERS, 1999, 24 (02) :113-115

[8] Raman and CARS microspectroscopy of cells and tissues [J].

Krafft, Christoph ;

Dietzek, Benjamin ;

Popp, Juergen .

ANALYST, 2009, 134 (06) :1046-1057

[9] Compact coherent anti-Stokes Raman scattering microscope based on a picosecond two-color Er:fiber laser system [J].

Krauss, Guenther ;

Hanke, Tobias ;

Sell, Alexander ;

Traeutlein, Daniel ;

Leitenstorfer, Alfred ;

Selm, Romedi ;

Winterhalder, Martin ;

Zumbusch, Andreas .

OPTICS LETTERS, 2009, 34 (18) :2847-2849

[10] High power red and near-IR generation using four wave mixing in all integrated fibre laser systems [J].

Lavoute, Laure ;

Knight, Jonathan C. ;

Dupriez, Pascal ;

Wadsworth, William J. .

OPTICS EXPRESS, 2010, 18 (15) :16193-16205

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