Binding mode analysis of 3-(4-benzoyl-1-methyl-1H-2-pyrrolyl)-N-hydroxy-2-propenamide:: A new synthetic histone deacetylase inhibitor inducing histone hyperacetylation, growth inhibition, and terminal cell differentiation

被引:60
作者
Mai, A
Massa, S
Ragno, R
Esposito, M
Sbardella, G
Nocca, G
Scatena, R
Jesacher, F
Loidl, P
Brosch, G
机构
[1] Univ Roma La Sapienza, Dipartimento Studi Farmaceut, I-00185 Rome, Italy
[2] Univ Siena, Dipartimento Farmaco Chim Tecnol, I-53100 Siena, Italy
[3] Univ Roma La Sapienza, Dipartimento Studi Chim & Tecnol Sostanze Biologi, I-00185 Rome, Italy
[4] Univ Salerno, Dipartimento Sci Farmaceut, I-84084 Fisciano, SA, Italy
[5] Univ Cattolica Sacro Cuore, Ist Biochim & Biochim Clin, I-00168 Rome, Italy
[6] Univ Innsbruck, Sch Med, Dept Microbiol, A-6020 Innsbruck, Austria
关键词
D O I
10.1021/jm011088+
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
The binding mode of 3-(4-aroyl-1H-2-pyrrolyl)-N-hydroxy-2-propenamides 1a-c, belonging to a recently reported class of synthetic histone deacetylase (HDAC) inhibitors (Massa, S.; et al. J. Med. Chem. 2001, 44, 2069-2072), into the new modeled HDAC 1 catalytic core is presented, and enzyme/inhibitor interactions are discussed. HDAC1 X-ray coordinates were obtained by virtual "mutation" of those of histone deacetylase-like protein, a bacterial HDAC homologue. In in vitro antimaize HD2 as well as antimouse HDAC1 assay, compounds 1a-c showed inhibitory activities in the low micromolar range. Similarly, 1a-C are endowed with anti-HDAC activity in vivo: on mouse A20 cells, 1a-c induced histone hyperacetylation leading to a highly increased acetylation level of H4 as compared to control histones. Results obtained with acid-urea-triton polyacrylamide gel electrophoresis have been confirmed by Western Blot experiments. Finally, compound 1a, chosen as a representative member of this class of HDAC inhibitors, resulted endowed with antiproliferative (45 and 85% cell growth inhibition at 40 and 80 muM, respectively) and cellular differentiation (18 and 21% of benzidine positive cells at the same concentrations) activities in murine erythroleukemic cells.
引用
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页码:1778 / 1784
页数:7
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