Comparative proteomic analysis of human mesenchymal and embryonic stem cells: Towards the definition of a mesenchymal stem cell proteomic signature

被引：69

作者：

Roche, Stephane ^{[1
,2
]}

Delorme, Bruno ^{[3
]}

Oostendorp, Robert A. J. ^{[4
]}

Barbet, Romain ^{[5
]}

Caton, David ^{[6
]}

Noel, Daniele ^{[7
,8
]}

Boumediene, Karim ^{[9
]}

Papadaki, Helen A. ^{[10
]}

Cousin, Beatrice ^{[6
]}

Crozet, Carole ^{[1
]}

Milhavet, Ollivier ^{[1
]}

Casteilla, Louis ^{[6
]}

Hatzfeld, Jacques ^{[5
]}

Jorgensen, Christian ^{[7
,8
]}

Charbord, Pierre ^{[3
]}

Lehmann, Sylvain ^{[1
,2
,8
]}

机构：

[1] CNRS, Inst Genet Humaine, UPR1142, F-34000 Montpellier, France

[2] CHU Montpellier, Hop St Eloi, Biochim Lab, Montpellier, France

[3] Fac Med Tours, INSERM, ESPRI, Lab Hematopoiese,EA 3855, Tours, France

[4] Tech Univ Munich, Klinikum Rechts Isar, Dept Internal Med 3, D-8000 Munich, Germany

[5] CNRS, UPR 9045, Inst Andre Lwoff, Villejuif, France

[6] CNRS, UMR 5241, UPS, Inst Louis Bugnard, Toulouse, France

[7] INSERM, U844, Montpellier, France

[8] Univ Montpellier I, Montpellier, France

[9] Univ Caen Basse Normandie, UFR Med, EA3214, Lab Matrice Extracellulaire & Pathol, Caen, France

[10] Univ Crete, Sch Med, Dept Hematol, Iraklion, Crete, Greece

来源：

PROTEOMICS | 2009年 / 9卷 / 02期

关键词：

Adipose tissue; Bone marrow; Embryonic stem cell; Mesenchymal stem cell; Umbilical vein; UMBILICAL-CORD BLOOD; HUMAN BONE-MARROW; ADIPOSE-TISSUE; STROMAL CELLS; IDENTIFICATION; SYNOVIUM;

D O I：

10.1002/pmic.200800035

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mesenchymal stem cells (MSC) are adult multipotential progenitors which have a high potential in regenerative medicine. They can be isolated from different tissues throughout the body and their homogeneity in terms of phenotype and differentiation capacities is a real concern. To address this issue, we conducted a 2-DE gel analysis of mesenchymal stem cells isolated from bone marrow (BM), adipose tissue, synovial membrane and umbilical vein wall. We confirmed that BM and adipose tissue derived cells were very similar, which argue for their interchangeable use for cell therapy. We also compared human mesenchymal to embryonic stem cells and showed that umbilical vein wall stem cells, a neo-natal cell type, were closer to BM cells than to embryonic stem cells. Based on these proteomic data, we could propose a panel of proteins which were the basis for the definition of a mesenchymal stem cell proteomic signature.

引用

页码：223 / 232

页数：10

共 27 条

[1] Identification of mesenchymal progenitor cells in normal and osteoarthritic human articular cartilage [J].

Alsalameh, S ;

Amin, R ;

Gemba, T ;

Lotz, M .

ARTHRITIS AND RHEUMATISM, 2004, 50 (05) :1522-1532

[2] Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells [J].

Colter, DC ;

Sekiya, I ;

Prockop, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (14) :7841-7845

[3]

De Bari C, 2001, ARTHRITIS RHEUM-US, V44, P1928, DOI 10.1002/1529-0131(200108)44:8<1928::AID-ART331>3.0.CO

[4]

2-P

[5] Proteomic analysis of primary cultures of human adipose-derived stem cells - Modulation by adipogenesis [J].

DeLany, JP ;

Floyd, ZE ;

Zvonic, S ;

Smith, A ;

Gravois, A ;

Reiners, E ;

Wu, XY ;

Kilroy, G ;

Lefevre, M ;

Gimble, JM .

MOLECULAR & CELLULAR PROTEOMICS, 2005, 4 (06) :731-740

[6] Transcriptional profiles discriminate bone marrow-derived and synovium-derived mesenchymal stem cells [J].

Djouad, F ;

Bony, C ;

Häupl, T ;

Uzé, G ;

Lahlou, N ;

Louis-Plence, P ;

Apparailly, F ;

Canovas, F ;

Rème, T ;

Sany, J ;

Jorgensen, C ;

Noël, D .

ARTHRITIS RESEARCH & THERAPY, 2005, 7 (06) :R1304-R1315

[7] Stem cell proteomes:: A profile of human mesenchymal stem cells derived from umbilical cord blood [J].

Feldmann, RE ;

Bieback, K ;

Maurer, MH ;

Kalenka, A ;

Bürgers, HF ;

Gross, B ;

Hunzinger, C ;

Klüter, H ;

Kuschinsky, W ;

Eichler, H .

ELECTROPHORESIS, 2005, 26 (14) :2749-2758

[8] Development of regenerative cardiomyocytes from mesenchymal stem cells for cardiovascular tissue engineering [J].

Fukuda, K .

ARTIFICIAL ORGANS, 2001, 25 (03) :187-193

[9] Proteomic analysis of human bone marrow mesenchymal stem cells transduced with human telomerase reverse transcriptase gene during proliferation [J].

Huang, G. P. ;

Pan, Z. J. ;

Huang, J. P. ;

Yang, J. F. ;

Guo, C. J. ;

Wang, Y. G. ;

Zheng, Q. ;

Chen, R. ;

Xu, Y. L. ;

Wang, G. Z. ;

Xi, Y. M. ;

Shen, D. ;

Jin, J. ;

Wang, J. F. .

CELL PROLIFERATION, 2008, 41 (04) :625-644

[10] Do adipose tissue-derived mesenchymal stem cells have the same osteogenic and chondrogenic potential as bone marrow-derived cells? [J].

Im, GI ;

Shin, YW ;

Lee, KB .

OSTEOARTHRITIS AND CARTILAGE, 2005, 13 (10) :845-853

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