Effect of the spacer arm on non-specific binding in membrane affinity chromatography

被引：7
|
作者：
Lalli, Eleonora [1 ]
Sarti, Giulio C. [1 ]
Boi, Cristiana [1 ]
机构：
[1] Alma Mater Studiorum Univ Bologna, DICAM, Dipartimento Ingn Civile Chim Ambientale & Mat, Via Terracini 28, I-40131 Bologna, Italy
来源：
MRS COMMUNICATIONS | 2018年 / 8卷 / 01期
关键词：
PURIFICATION; ANTIBODIES; LIGAND;
D O I：
10.1557/mrc.2018.4
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
The preparation, screening, and characterization of affinity membranes require a deep knowledge of the behavior of all components of the affinity material. Several studies report the effect of different spacers in combination with the ligand molecule, but the effect of the spacer arm per se is generally disregarded. The effect of the spacer 1,2-diaminoethane on non-specific protein adsorption was recently investigated and the results were compared with the ones obtained with A2P affinity membranes. The results show that this spacer has indeed an important effect and that similar specific studies need to be performed for every spacer molecule.
引用
收藏
页码：65 / 70
页数：6
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