Involvement of eIF2α of Epinephelus coioides in the fish immune response to virus infection

被引：3

作者：

Zang, Shaoqing ^{[1
,2
,5
]}

Zhang, Xin ^{[3
]}

Zhang, Jingcheng ^{[1
,2
,5
]}

Li, Chen ^{[3
]}

Wei, Jingguang ^{[3
]}

Qin, Qiwei ^{[1
,2
,3
,4
,5
]}

机构：

[1] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China

[4] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China

[5] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China

来源：

FISH & SHELLFISH IMMUNOLOGY | 2018年 / 75卷

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

elF2; alpha; Phosphorylation; Epinephelus coioides; RGNNV; DEPENDENT PROTEIN-KINASE; ORANGE-SPOTTED GROUPER; INITIATION FACTOR-2-ALPHA KINASE; ENDOPLASMIC-RETICULUM STRESS; NF-KAPPA-B; FUNCTIONAL-ANALYSIS; MOLECULAR-CLONING; BINDING DOMAINS; ALPHA-SUBUNIT; ER STRESS;

D O I：

10.1016/j.fsi.2018.02.027

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The eukaryotic initiation factor 2 alpha subunit (eIF2 alpha) is a key translation regulator that plays an important role in different cellular pressures and stimuli, including virus infection. In the present study, an eIF2 alpha homolog (EcelF2 alpha) from the orange-spotted grouper (Epinephelus coioides) was cloned and its roles during fish viral infection were characterized. EcelF2 alpha encodes a putative protein of 315 amino acid residues, and shares a high degree of similarity with elF2 alpha s from other species. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that EcelF2 alpha was distributed in all examined tissues. Both of the expression levels of EceIF2 alpha in the spleen and head kidney of E. coioides were up-regulated when challenged with polyinosine-polycytidylic acid (poly[I:C]). EceIF2 alpha was abundantly distributed in both the cytoplasm and nucleus in grouper spleen (GS) cells. Over-expression of EceIF2 alpha improved the expression of red-spotted grouper nervous necrosis virus (RGNNV) genes in GS cells. In addition, EceIF2 alpha depressed the activation of NK-kappa B and IFN-beta. Furthermore, dephosphorylation inhibitor treatment led to a significant decrease of RGNNV gene transcription. Taken together, these results suggest that EcelF2 alpha might be involved in the fish immune response to virus challenge.

引用

页码：365 / 373

页数：9

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