Coordinate regulation of RAG1 and RAG2 by cell type-specific DNA elements 5′ of RAG2

被引：119
|
作者：
Yu, W
Misulovin, Z
Suh, H
Hardy, RR
Jankovic, M
Yannoutsos, N
Nussenzweig, MC
机构：
[1] Rockefeller Univ, Howard Hughes Med Inst, Lab Mol Immunol, New York, NY 10021 USA
[2] Fox Chase Canc Ctr, Inst Canc Res, Philadelphia, PA 19111 USA
来源：
SCIENCE | 1999年 / 285卷 / 5430期
关键词：
D O I：
10.1126/science.285.5430.1080
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
RAG1 and RAG2 are essential for V(D)J recombination and Lymphocyte development. These genes are thought to encode a transposase derived from a mobile genetic element that was inserted into the vertebrate genome 450 million years ago. The regulation of RAG1 and RAG2 was investigated in vivo with bacterial artificial chromosome (BAC) transgenes containing a fluorescent indicator. Coordinate expression of RAG1 and RAG2 in B and T cells was found to be regulated by distinct genetic elements found on the 5' side of the RAG2 gene. This observation suggests a mechanism by which asymmetrically disposed cis DNA elements could influence the expression of the primordial transposon and thereby capture RAGs for vertebrate evolution.
引用
收藏
页码：1080 / 1084
页数：5
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