A High-throughput Platform for the Production and Analysis of Transgenic Cassava (Manihot esculenta) Plants

被引：65

作者：

Taylor, Nigel ^{[1
]}

Gaitan-Solis, Eliana ^{[1
]}

Moll, Theodore ^{[1
]}

Trauterman, Brent ^{[2
]}

Jones, Tira ^{[1
]}

Pranjal, Amita ^{[1
]}

Trembley, Cindy ^{[1
]}

Abernathy, Vince ^{[1
]}

Corbin, David ^{[3
]}

Fauquet, Claude M. ^{[1
]}

机构：

[1] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA

[2] Univ Texas SW Med Ctr Dallas, Dallas, TX 75390 USA

[3] Dow Agro Sci, Indianapolis, IN USA

来源：

TROPICAL PLANT BIOLOGY | 2012年 / 5卷 / 01期

基金：

比尔及梅琳达.盖茨基金会;

关键词：

Cassava; DNA genomic dot blot; Friable embryogenic callus; Genetic transformation;

D O I：

10.1007/s12042-012-9099-4

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A platform for high-throughput production and analysis of transgenic cassava (Manihot esculenta) has been developed for the variety 60444 and implemented to generate plants expressing traits for nutritional enhancement, modified metabolism, promoter analysis and disease resistance. Over a three and a half year period this system has been utilized to produce more than 3500 independent transgenic plant lines from 50 different genetic constructs within a single laboratory. Plants recovered through this system have proven robust and efficacious for engineered traits under greenhouse conditions and within the first confined field trials of transgenic cassava carried out in Uganda, Kenya, Nigeria and Puerto Rico. Detailed procedures are described for the operation of this platform, including all steps in tissue culture, genetic transformation, copy number estimation, greenhouse establishment for shoot and storage root formation and systems for centralized quality control, trans-genic plant tracking and regulatory compliance. In addition to providing reliable transgenic plant production for proof of concept studies in the variety 60444, the systems implemented and described here form the structure for high throughput production of transgenic farmer-preferred cultivars of cassava.

引用

页码：127 / 139

页数：13

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