Stallion spermatozoa selected by single layer centrifugation are capable of fertilization after storage for up to 96 h at 6°C prior to artificial insemination

被引：21

作者：

Lindahl, Johanna ^{[1
]}

Dalin, Anne-Marie ^{[1
]}

Stuhtmann, Gesa ^{[2
]}

Morrell, Jane M. ^{[1
]}

机构：

[1] Swedish Univ Agr Sci, Dept Reprod, SLU, Uppsala, Sweden

[2] Flyinge AB, S-24729 Flyinge, Sweden

来源：

ACTA VETERINARIA SCANDINAVICA | 2012年 / 54卷

关键词：

Single layer centrifugation; Stored stallion spermatozoa; Fertilizing capability; Artificial insemination; DENSITY GRADIENT; SEMEN; EJACULATE; OVULATION; MOTILITY; FRESH;

D O I：

10.1186/1751-0147-54-40

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Background: One of the challenges faced by equine breeders is ensuring delivery of good quality semen doses for artificial insemination when the mare is due to ovulate. Single Layer Centrifugation (SLC) has been shown to select morphologically normal spermatozoa with intact chromatin and good progressive motility from the rest of the ejaculate, and to prolong the life of these selected spermatozoa in vitro. The objective of the present study was a proof of concept, to determine whether fertilizing ability was retained in SLC-selected spermatozoa during prolonged storage. Findings: Sixteen mares were inseminated with SLC-selected sperm doses that had been cooled and stored at 6 degrees C for 48 h, 72 h or 96 h. Embryos were identified in 11 mares by ultrasound examination 16-18 days after presumed ovulation. Conclusion: SLC-selected stallion spermatozoa stored for up to 96 h are capable of fertilization.

引用

页数：5

共 17 条

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