P38-associated pathway involvement in apoptosis induced by photodynamic therapy with Lonicera japonica in human lung squamous carcinoma CH27 cells

被引：44

作者：

Leung, Henry W. -C. ^{[2
]}

Hour, M. -J. ^{[1
]}

Chang, W. -T. ^{[3
]}

Wu, Y. -C. ^{[4
]}

Lai, M. -Y. ^{[1
]}

Wang, M. -Y. ^{[1
]}

Lee, H. -Z. ^{[1
]}

机构：

[1] China Med Univ, Sch Pharm, Taichung 40402, Taiwan

[2] Chi Mei Hosp, Dept Radiat Oncol, Tainan, Taiwan

[3] China Med Univ, Sch Chinese Med Resources, Taichung, Taiwan

[4] Kaohsiung Med Univ, Sch Pharm, Grad Inst Nat Prod, Kaohsiung, Taiwan

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2008年 / 46卷 / 11期

关键词：

Photodynamic therapy; Lonicera japonica; Apoptosis; Lung CH27 carcinoma cells; P38; F-actin;

D O I：

10.1016/j.fct.2008.08.022

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Photodynamic therapy (PDT) is an effective therapy for local malignant tumors. Lonicera japonica was found to have the anti-tumor effect. The aim of this study is to explore the mechanisms of apoptosis induced by PDT in lung CH27 carcinoma cells with alcohol extract from Lonicera japonica as photosensitizer. Our study indicated that Lonicera japonica extracts exhibited significant photocytotoxicity in CH27 cells at a concentration range of 50-150 mu g/ml, with 0.4-1.2 J/cm(2) light dose. PDT with Lonicera japonica extracts-induced cell death is a typical apoptosis that was accompanied by DNA condensation, externalization of phosphatidylserine and formation of apoptotic bodies. PDT with Lonicera japonica extracts was shown to be caspase-3-independent apoptosis via activation of AIF in this study. P38-associated pathway may be involved in apoptosis induced by PDT with Lonicera japonica extracts in CH27 cells. We also have demonstrated that PDT with Lonicera japonica extracts-induced CH27 cells apoptosis was probably related to its ability to change the protein expression and distribution of heat shock protein 27. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：3389 / 3400

页数：12

共 25 条

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