Sodium fluoride disturbs DNA methylation of NNAT and declines oocyte quality by impairing glucose transport in porcine oocytes

被引：28

作者：

Liu, Xiaoyan ^{[1
,2
,3
]}

Nie, Zheng-Wen ^{[1
,2
,4
]}

Gao, Ying-Ying ^{[1
,2
,4
]}

Chen, Li ^{[1
,2
,4
]}

Yin, Shu-Yuan ^{[1
,2
,4
]}

Zhang, Xia ^{[1
,2
,5
]}

Hao, Cuifang ^{[3
]}

Miao, Yi-Liang ^{[1
,2
,4
,5
]}

机构：

[1] Huazhong Agr Univ, Coll Anim Sci & Technol, Inst Stem Cell & Regenerat Biol, Wuhan 430070, Hubei, Peoples R China

[2] Huazhong Agr Univ, Coll Vet Med, Wuhan 430070, Hubei, Peoples R China

[3] Qingdao Med Univ, Yuhuangding Hosp Yantai, Affiliated Hosp, Reprod Med Ctr, Yantai, Shandong, Peoples R China

[4] Huazhong Agr Univ, Minist Educ, Key Lab Agr Anim Genet Breeding & Reprod, Wuhan 430070, Hubei, Peoples R China

[5] Cooperat Innovat Ctr Sustainable Pig Prod, Wuhan 430070, Hubei, Peoples R China

来源：

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS | 2018年 / 59卷 / 03期

关键词：

methylation; NNAT; oocyte; pig; sodium fluoride; ADENYLATE-CYCLASE STIMULATION; BOVINE OOCYTES; MOUSE OOCYTES; FEMALE MICE; APOPTOSIS; FERTILIZATION; RESUMPTION; MATURATION; EXPOSURE; EMBRYOS;

D O I：

10.1002/em.22165

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Sodium fluoride (NaF) is used as a medicine to prevent tooth decay; however, excessive NaF could cause a pathological damage to the health. Recent studies showed that NaF impaired mouse oocyte maturation, included of abnormal spindle configuration, actin cap formation, cortical granule-free domain formation, and the following development after fertilization. However, few studies used large animals as models to study the toxicology of NaF on oocytes maturation. We proposed a hypothesis that NaF would affect the nuclear and cytoplasmic maturation of porcine oocytes and DNA methylation pattern of imprinted genes in oocytes. Our results showed that NaF affected cumulus expansion, polar body emission, spindle morphology, cortical granule distribution, early apoptosis, and the following development after parthenogenetic activation during porcine oocyte maturation. Moreover, NaF increased the DNA methylation of NNAT and decreased its expression, which disturbed the glucose transport in oocytes. These results suggest that NaF impairs the porcine oocytes maturation epigenetically, which provides a new toxicological mechanism of NaF on the oocyte maturation. Environ. Mol. Mutagen. 59:223-233, 2018. (c) 2017 Wiley Periodicals, Inc.

引用

页码：223 / 233

页数：11