A simple "mix-and-detection" method for the sensitive detection of telomerase from cancer cells under absolutely isothermal conditions

被引：41
|
作者：
Ma, Fei [1 ]
Wei, Shu-hua [1 ]
Leng, Junhong [2 ]
Tang, Bo [1 ]
Zhang, Chun-yang [1 ]
机构：
[1] Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Collaborat Innovat Ctr Functionalized Probes Chem, Key Lab Mol & Nano Probes,Minist Educ,Shandong Pr, Jinan 250014, Shandong, Peoples R China
[2] Jinan Matern & Child Care Hosp, Jinan 250000, Shandong, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2018年 / 54卷 / 20期
基金：
中国国家自然科学基金;
关键词：
ROLLING-CIRCLE AMPLIFICATION; TARGET RECYCLING AMPLIFICATION; REAL-TIME; SIGNAL AMPLIFICATION; INTRACELLULAR TELOMERASE; FLUORESCENCE DETECTION; GOLD NANOPARTICLES; MOLECULAR BEACON; NUCLEIC-ACIDS; ASSAY;
D O I：
10.1039/c8cc00093j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We develop a simple mix-and-detection method for the sensitive detection of telomerase from cancer cells under absolutely isothermal conditions. This method enables one-step and one-tube detection of telomerase with a detection limit of 3 cells, and it can be further applied for the screening of telomerase inhibitors and the discrimination of cancer cells from normal cells.
引用
收藏
页码：2483 / 2486
页数：4
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