HER-2 gene amplification by fluorescence in situ hybridization (FISH) compared with immunohistochemistry (IHC) in breast cancer: a study of 528 equivocal cases

被引：19
作者：
Zhang, Han [1 ]
Ren, Guoping [1 ]
Wang, Xiaoling [1 ]
Zhao, Jing [1 ]
Yao, Hongtian [1 ]
Bai, Yanfeng [1 ]
Bo, Wang [1 ]
机构：
[1] Zhejiang Univ, Dept Pathol, Affiliated Hosp 1, Hangzhou 310003, Zhejiang, Peoples R China
来源：
BREAST CANCER RESEARCH AND TREATMENT | 2012年 / 134卷 / 02期
基金：
匈牙利科学研究基金会;
关键词：
Breast cancer; Fluorescence in situ hybridization; HER-2/neu; PROGESTERONE-RECEPTOR GENE; INTEROBSERVER REPRODUCIBILITY; PROTEIN OVEREXPRESSION; ESTROGEN; EXPRESSION; ANEUSOMY; TISSUE;
D O I：
10.1007/s10549-012-2101-x
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The concordance rate between fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) on evaluating HER-2/neu status is still controversial, especially for the IHC 2+/equivocal cases. In this study, we investigated the amplification of HER-2/neu gene by FISH in IHC (2+)/equivocal specimens to clarify the correlation between HER-2/neu and clinicopathologic features of breast cancers. HER-2/neu amplification was determined by FISH on 528 specimens of breast carcinomas with equivocal expression of HER-2/neu protein by IHC detection. 65.5 % of IHC 2+ patients were negative for HER-2/neu amplification, 29.0 % were positive and the remaining was equivocal. A statistically significant inverse association was found between hormone receptor expression and HER-2/neu amplification (P < 0.05). Furthermore, polysomy of CEP17 was detected in 60 % of breast carcinomas. The results highlight the necessity of FISH test for further categorization when breast carcinoma cases are scored 2+ by IHC.
引用
收藏
页码：743 / 749
页数：7
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