A superoxide anion electrochemical sensor based on the direct electrochemistry of superoxide dismutase assembled layer-by-layer at the L-cysteine modified gold electrode

被引：0

作者：

Yang, Yan ^{[1
]}

Zhou, Jiawan ^{[1
]}

Wu, Liang ^{[1
]}

Zhang, Xiaohua ^{[1
]}

Chen, Jinhua ^{[1
]}

机构：

[1] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China

来源：

INDIAN JOURNAL OF CHEMISTRY SECTION A-INORGANIC BIO-INORGANIC PHYSICAL THEORETICAL & ANALYTICAL CHEMISTRY | 2012年 / 51卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Superoxide anion; Superoxide dismutase; Electrochemistry; Poly-(diallyldimethylammonium); Sensors; Modified electrodes; Layer-by-layer assembly; 3RD-GENERATION BIOSENSOR; HORSERADISH-PEROXIDASE; GLUCOSE-OXIDASE; NANOPARTICLES; FILMS; COPPER; HEMOGLOBIN; PROTEINS; NANORODS; SOD;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A superoxide anion (O-2(center dot-)) biosensor has been developed based on layer-by-layer assembled poly-(diallyldimethylammonium) (PDDA) and superoxide dismutase (SOD) on the L-cysteine (Cys) modified gold (Au) electrode. In 25 mM phosphate buffer solution (pH 7.2), the {SOD/PDDA}(n)/Cys/Au electrode shows a pair of well-defined and nearly reversible peak at similar to 84 mV vs SCE, confirming the direct electrochemistry of SOD. Based on the bifunctional enzymatic catalytic activities of SOD to the oxidation and reduction of O-2(center dot-), the developed {SOD/PDDA}(5)/Cys/Au electrode exhibits good analytical characteristics in the detection of O-2(center dot-), such as low detection limit, good stability, reproducibility, selectivity and particularly a wide linear range (0.5 similar to 546 mu M).

引用

页码：1057 / 1063

页数：7

共 35 条

[1]

[Anonymous], ANAL CHIM ACTA

[2]

[Anonymous], SCI CHINA B

[3]

[Anonymous], ELECTROANAL

[4] What is the ultimate fate of superoxide anion in vivo? [J].