High-Salt Stress Conditions Increase the pAW63 Transfer Frequency in Bacillus thuringiensis

被引：25

作者：

Beuls, Elise ^{[1
]}

Modrie, Pauline ^{[1
]}

Deserranno, Cedric ^{[1
]}

Mahillon, Jacques ^{[1
]}

机构：

[1] Catholic Univ Louvain, Lab Food & Environm Microbiol, B-1348 Louvain, Belgium

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2012年 / 78卷 / 19期

关键词：

SUBSP ISRAELENSIS; CEREUS GROUP; PLASMID TRANSFER; LABORATORY CULTURE; SUBTILIS; CONJUGATION; MEMBERS; ADAPTATION; ANTHRACIS; KURSTAKI;

D O I：

10.1128/AEM.01105-12

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Conjugation experiments with Bacillus thuringiensis and transfer kinetics demonstrated that salt stress has a positive impact on plasmid transfer efficiency. Compared to standard osmotic conditions (0.5% NaCl), plasmid transfer occurred more rapidly, and at higher frequencies (>100-fold), when bacteria were exposed to a high-salt stress (5% NaCl) in liquid brain heart infusion (BHI). Under milder salt conditions (2.5% NaCl), only a 10-fold effect was observed in Luria-Bertani broth and no difference was detected in BHI. These observations are particularly relevant in the scope of potential gene exchanges among members of the Bacillus cereus group, which includes food-borne contaminants and pathogens.

引用

页码：7128 / 7131

页数：4

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