Immune responses of zebrafish (Danio rerio) induced by bath-vaccination with a live attenuated Vibrio anguillarum vaccine candidate

被引：72

作者：

Zhang, Zhihui ^{[1
]}

Wu, Haizhen ^{[1
]}

Xiao, Jingfan ^{[1
]}

Wang, Qiyao ^{[1
]}

Liu, Qin ^{[1
]}

Zhang, Yuanxing ^{[1
]}

机构：

[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

来源：

FISH & SHELLFISH IMMUNOLOGY | 2012年 / 33卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Bath-vaccination; Danio rerio; Immunoprotection; Live attenuated vaccine; Vibrio anguillarum; TROUT ONCORHYNCHUS-MYKISS; MYCOBACTERIUM-MARINUM INFECTION; SEPTICEMIA VIRUS VHSV; RAINBOW-TROUT; YERSINIA-RUCKERI; ANTIBACTERIAL ACTIVITY; AEROMONAS-SALMONICIDA; EDWARDSIELLA-TARDA; ADAPTIVE IMMUNITY; ATLANTIC SALMON;

D O I：

10.1016/j.fsi.2012.03.031

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

A fish vaccine candidate, live attenuated Vibrio anguillarum, which can protect fish from vibriosis, was established in our laboratory. In this study, the protective immunological mechanism of live attenuated V. anguillarum was investigated in zebrafish as a model animal. After bath-vaccinated with the live attenuated strain, zebrafish were challenged with wild pathogenic strain to test the immunoprotection of the live attenuated strain. As the results, specific antibody response of fish against V. anguillarum was found to gradually increase during 28 days post-vaccination, and remarkable protection was showed with a high relative protection survival (RPS) of about 90%. Moreover, the vaccination changed the expressions of several immune-related genes in the spleens and livers of zebrafish. Among them, the expressions of pro-inflammatory factors such as IL-1 and IL-8 were tenderly up-regulated with about 3-4 fold in 1-7 days post-vaccination, while MHC II rose to a peak level of 4-fold in 7th day post-vaccination. These results gave some important messages about the mechanism of specific protection induced by live attenuated V. anguillarum and showed the availability of zebrafish model in the evaluation of the vaccine candidate. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：36 / 41

页数：6

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