Human cervical mucus can act in vitro as a selective barrier against spermatozoa carrying fragmented DNA and chromatin structural abnormalities

被引：19
作者：
Bianchi, PG
De Agostini, A
Fournier, J
Guidetti, C
Tarozzi, N
Bizzaro, D
Manicardi, GC
机构：
[1] Univ Modena & Reggio Emilia, Dipartimento Sci Agr, I-42100 Reggio Emilia, Italy
[2] Univ Hosp Geneva, Dept Obstet & Gynaecol, Clin Steril, Geneva, Switzerland
[3] Univ Politecn Marche, Ist Biol & Genet, Ancona, Italy
来源：
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 2004年 / 21卷 / 04期
关键词：
chromatin abnormalities; chromomycin A(3); human cervical mucus; in situ nick translation; spermatozoa;
D O I：
10.1023/B:JARG.0000029492.54243.3c
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Purpose: We have carried out experiments to determine if human cervical mucus can act as an in vitro selective barrier against spermatozoa morphologically normal that carry genetic structural abnormalities. Methods: Sperm chromatin abnormalities have been evaluated by Chromomycin A(3) and "endogenous" nick translation. Results: The data obtained have shown that spermatozoa possessing higher levels of DNA protamination are more proficient in crossing the cervical mucus barrier. Moreover, the levels of positivity to endogenous nick translation treatment was practically zero in such spermatozoa. Conclusions: We suggest that sperm penetration of cervical mucus could be used to select sperm preparations free of fragmented DNA or chromatin structural abnormalities for assisted reproduction.
引用
收藏
页码：97 / 102
页数：6
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