Portable-surface plasmon resonance biosensor immunoassays for the human serum albumin detection

被引：3

作者：

Li, Hui ^{[1
]}

Zhang, Lu-lu ^{[1
]}

Cai, Hao-yuan ^{[1
]}

Chen, Xing ^{[1
]}

Sun, Jian-hai ^{[1
]}

Chao, Ya-peng ^{[2
]}

Cui, Da-fu ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Elect, State Key Lab Transducer Tech, Beijing 100190, Peoples R China

[2] Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China

来源：

MICRO-NANO TECHNOLOGY XIV, PTS 1-4 | 2013年 / 562-565卷

基金：

国家高技术研究发展计划(863计划);

关键词：

surface plasmon resonance biosensor; human serum albumin; detection; EXCRETION; IMMUNONEPHELOMETRY;

D O I：

10.4028/www.scientific.net/KEM.562-565.408

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The screening of urine human serum albumin (HSA) is significant for the early diagnosis and intervention of the nephropathia. This study developed sensitive HSA detection assay with a self-made portable-SPR biosensor based on the reflection light intensity interrogation. Anti-HSA monoclonal antibody was immobilized onto the gold sensing area and a directive assay for HSA was developed. A detection range of 1-100 mu g/mL USA was found. The described label-free, real-time immunoassay for the quantification of HSA in urine is a rapid, sensitive, alternative method available for the clinic detection.

引用

页码：408 / +

页数：2

共 8 条

[1] Which method for quantifying urinary albumin excretion gives what outcome? A comparison of immunonephelometry with HPLC [J].

Brinkman, JW ;

Bakker, SJL ;

Gansevoort, RT ;

Hillege, HL ;

Kema, IP ;

Gans, ROB ;

De Jong, PE ;

De Zeeuw, D .

KIDNEY INTERNATIONAL, 2004, 66 :S69-S75

[2] Portable Surface Plasmon Resonance Instrument based on a Monolithic Scanner Chip [J].

Cai, Haoyuan ;

Li, Hui ;

Zhang, Lulu ;

Chen, Xing ;

Cui, Dafu .

2010 3RD INTERNATIONAL CONFERENCE ON BIOMEDICAL ENGINEERING AND INFORMATICS (BMEI 2010), VOLS 1-7, 2010, :1553-1555

[3]

GIAMPIETRO O, 1987, J NUCL MED ALLIED S, V31, P321

[4] IMMOBILIZATION OF PROTEINS TO A CARBOXYMETHYLDEXTRAN-MODIFIED GOLD SURFACE FOR BIOSPECIFIC INTERACTION ANALYSIS IN SURFACE-PLASMON RESONANCE SENSORS [J].

JOHNSSON, B ;

LOFAS, S ;

LINDQUIST, G .

ANALYTICAL BIOCHEMISTRY, 1991, 198 (02) :268-277

[5]

MARRE M, 1987, CLIN CHEM, V33, P209

[6] Immunosensor for the measurement of human serum albumin in urine based on the Spreeta surface plasmon resonance sensor [J].

Navrátilová, I ;

Skládal, P .

SUPRAMOLECULAR CHEMISTRY, 2003, 15 (02) :109-115

[7] Development of piezoelectric immunosensors for measurement of albuminuria [J].

Navrátilová, I ;

Skládal, P ;

Viklicky, V .

TALANTA, 2001, 55 (04) :831-839

[8]

VIBERTI GC, 1982, LANCET, V1, P1430

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