Hydrogen Peroxide Produced by Oral Streptococci Induces Macrophage Cell Death

被引:36
|
作者
Okahashi, Nobuo [1 ]
Nakata, Masanobu [2 ]
Sumitomo, Tomoko [2 ]
Terao, Yutaka [3 ]
Kawabata, Shigetada [2 ]
机构
[1] Osaka Univ, Grad Sch Dent, Dept Oral Frontier Biol, Suita, Osaka, Japan
[2] Osaka Univ, Grad Sch Dent, Dept Oral & Mol Microbiol, Suita, Osaka, Japan
[3] Niigata Univ, Grad Sch Med & Dent Sci, Div Microbiol & Infect Dis, Niigata, Japan
来源
PLOS ONE | 2013年 / 8卷 / 05期
基金
日本学术振兴会;
关键词
IDENTIFICATION; SANGUINIS; ENDOCARDITIS; VIRULENCE; PILI;
D O I
10.1371/journal.pone.0062563
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Hydrogen peroxide (H2O2) produced by members of the mitis group of oral streptococci plays important roles in microbial communities such as oral biofilms. Although the cytotoxicity of H2O2 has been widely recognized, the effects of H2O2 produced by oral streptococci on host defense systems remain unknown. In the present study, we investigated the effect of H2O2 produced by Streptococcus oralis on human macrophage cell death. Infection by S. oralis was found to stimulate cell death of a THP-1 human macrophage cell line at multiplicities of infection greater than 100. Catalase, an enzyme that catalyzes the decomposition of H2O2, inhibited the cytotoxic effect of S. oralis. S. oralis deletion mutants lacking the spxB gene, which encodes pyruvate oxidase, and are therefore deficient in H2O2 production, showed reduced cytotoxicity toward THP-1 macrophages. Furthermore, H2O2 alone was capable of inducing cell death. The cytotoxic effect seemed to be independent of inflammatory responses, because H2O2 was not a potent stimulator of tumor necrosis factor-a production in macrophages. These results indicate that streptococcal H2O2 plays a role as a cytotoxin, and is implicated in the cell death of infected human macrophages.
引用
收藏
页数:6
相关论文
共 50 条
  • [31] Assessment of cell death studies by monitoring hydrogen peroxide in cell culture
    Hirsch, Irina
    Prell, Erik
    Weiwad, Matthias
    ANALYTICAL BIOCHEMISTRY, 2014, 456 : 22 - 24
  • [32] Hydrogen peroxide induces endothelial cell atypia and cytoskeleton depolymerization
    Valen, G
    Sondén, A
    Vaage, J
    Malm, E
    Kjellström, BT
    FREE RADICAL BIOLOGY AND MEDICINE, 1999, 26 (11-12) : 1480 - 1488
  • [33] Hydrogen peroxide induces apoptosis-like death in Entamoeba histolytica trophozoites
    Nandi, Nilay
    Sen, Abhik
    Banerjee, Rajdeep
    Kumar, Sudeep
    Kumar, Vikash
    Ghosh, Amar Nath
    Das, Pradeep
    MICROBIOLOGY-SGM, 2010, 156 : 1926 - 1941
  • [34] Hydrogen peroxide induces apoptosis-like death in Leishmania donovani promastigotes
    Das, M
    Mukherjee, SB
    Shaha, C
    JOURNAL OF CELL SCIENCE, 2001, 114 (13) : 2461 - 2469
  • [35] Salivary hydrogen peroxide produced by holding or chewing green tea in the oral cavity
    Lambert, Joshua D.
    Kwon, Seok-Joo
    Hong, Jungil
    Yang, Chung S.
    FREE RADICAL RESEARCH, 2007, 41 (07) : 850 - 853
  • [36] Tanshinone IIA reduces macrophage death induced by hydrogen peroxide by upregulating glutathione peroxidase
    Li, Yu-I
    Elmer, Gary
    LeBoeuf, Renee C.
    LIFE SCIENCES, 2008, 83 (15-16) : 557 - 562
  • [37] ACIDS PRODUCED BY ORAL STREPTOCOCCI ON CEREAL SUBSTRATES
    STEINKRAUS, KH
    BIBBY, BG
    GILMOUR, MN
    JOURNAL OF DENTAL RESEARCH, 1969, 48 (5P2) : 928 - +
  • [38] PURIFICATION OF NEURAMINIDASES PRODUCED BY ORAL STREPTOCOCCI AND ACTINOMYCETES
    BAHN, A
    PRICE, P
    OTSUKA, A
    SUBBARAO, K
    HAYASHI, J
    JOURNAL OF DENTAL RESEARCH, 1981, 60 : 482 - 482
  • [39] Implication of hydrogen peroxide in the molecular mechanisms of apoptotic cell death
    Galaris, D.
    Amorgianiotis, C.
    Barbouti, A.
    FREE RADICAL RESEARCH, 2006, 40 : S149 - S149
  • [40] Hydrogen peroxide as a signal controlling plant programmed cell death
    Gechev, TS
    Hille, J
    JOURNAL OF CELL BIOLOGY, 2005, 168 (01): : 17 - 20