Visualization of human plasma fibrinogen adsorbed on highly oriented pyrolytic graphite by scanning probe microscopy

被引：26
|
作者：
Ohta, R [1 ]
Saito, N
Ishizaki, T
Takai, O
机构：
[1] Nagoya Univ, EcoTopia Sci Inst, Chikusa Ku, Nagoya, Aichi 4648603, Japan
[2] Nagoya Univ, Dept Mol Design & Engn, Chikusa Ku, Nagoya, Aichi 4648603, Japan
来源：
SURFACE SCIENCE | 2006年 / 600卷 / 08期
关键词：
atomic force microscopy; Kelvin probe force microscopy; phase image; fibrinogen-; highly oriented pyrolytic graphite;
D O I：
10.1016/j.susc.2005.12.058
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Human plasma fibrinogen (HPF) was observed by atomic force microscopy (AFM) and Kelvin probe force microscopy (KPFM) conducted in non-contact mode. The HPF was adsorbed on a highly oriented pyrolytic graphite (HOPG) substrate as single molecules, as aggregated bundles, and as aggregated fibers. Topographic and phase images confirmed structural changes in the HPF after exposure to air, while topographic and KPFM images confirmed fibers with the width of a single HPF molecule. Additionally, KPFM confirmed the surface potential difference between the HPF and the HOPG, and periodical potential drop reflecting the E and D domains in the fiber. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：1674 / 1678
页数：5
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