Poly(hydroxyethyl methacrylate) based magnetic nanoparticles for lysozyme purification from chicken egg white

被引：18
|
作者：
Kose, Kazim [1 ]
Denizli, Adil [1 ]
机构：
[1] Hacettepe Univ, Div Biochem, Dept Chem, Ankara, Turkey
来源：
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2013年 / 41卷 / 01期
关键词：
PHEMA; magnetic nanoparticles; lysozyme purification; affinity chromatography; GENERATION POLYMERIC NANOSPHERES; CIBACRON BLUE F3GA; AFFINITY PURIFICATION; HUMAN SERUM; IMMUNOGLOBULIN PRODUCTION; POLYSTYRENIC MATRICES; POLY(VINYL ALCOHOL); PROTEIN ADSORPTION; EFFICIENT REMOVAL; HYDROPHOBIC BEADS;
D O I：
10.3109/10731199.2012.696067
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The purpose of this article is to synthesize poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-tryptophan) [mPHEMATrp] magnetic nanoparticles for lysozyme purification from chicken egg white. mPHEMATrp nanoparticles (38 nm in diameter) were synthesized by surfactant-free emulsion polymerization. Specific surface area of the mPHEMATrp nanoparticles was calculated to be 896 m(2)/g. Elemental analysis of mPHEMATrp nanoparticles was estimated in the range of 5.7-48.3 mu mol MATrp/g. The maximum lysozyme adsorption capacity of the mPHEMATrp nanoparticles was 376.1 mg/g. The mPHEMATrp nanoparticles could be used five times without decreasing the lysozyme adsorption capacity significantly. The results indicate that the mPHEMATrp nanoparticles promise high selectivity for lysozyme purification.
引用
收藏
页码：13 / 20
页数：8
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