Whole Genome Sequence of Two Wild-Derived Mus musculus domesticus Inbred Strains, LEWES/EiJ and ZALENDE/EiJ, with Different Diploid Numbers

被引：9

作者：

Morgan, Andrew P. ^{[1
]}

Didion, John P. ^{[1
,4
]}

Doran, Anthony G. ^{[3
]}

Holt, James M. ^{[2
]}

McMillan, Leonard ^{[2
]}

Keane, Thomas M. ^{[3
]}

de Villena, Fernando Pardo-Manuel ^{[1
]}

机构：

[1] Univ N Carolina, Lineberger Comprehens Canc Ctr, Dept Genet, Chapel Hill, NC 27599 USA

[2] Univ N Carolina, Dept Comp Sci, Chapel Hill, NC 27599 USA

[3] Wellcome Trust Sanger Inst, Cambridge CB10 1HH, England

[4] NHGRI, NIH, Bethesda, MD 20892 USA

来源：

G3-GENES GENOMES GENETICS | 2016年 / 6卷 / 12期

基金：

英国生物技术与生命科学研究理事会; 英国医学研究理事会; 英国惠康基金; 美国国家卫生研究院;

关键词：

inbred mouse strains; wild-derived mouse strains; Robertsonian translocations; karyotype evolution; MOUSE STRAINS; HAPLOTYPE DIVERSITY; CONSOMIC STRAINS; GENE; MICE; DUPLICATION; KARYOTYPE; EVOLUTION;

D O I：

10.1534/g3.116.034751

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Wild-derived mouse inbred strains are becoming increasingly popular for complex traits analysis, evolutionary studies, and systems genetics. Here, we report the whole-genome sequencing of two wild-derived mouse inbred strains, LEWES/EiJ and ZALENDE/EiJ, of Mus musculus domesticus origin. These two inbred strains were selected based on their geographic origin, karyotype, and use in ongoing research. We generated 14x and 18x coverage sequence, respectively, and discovered over 1.1 million novel variants, most of which are private to one of these strains. This report expands the number of wild-derived inbred genomes in the Mus genus from six to eight. The sequence variation can be accessed via an online query tool; variant calls (VCF format) and alignments (BAM format) are available for download from a dedicated ftp site. Finally, the sequencing data have also been stored in a lossless, compressed, and indexed format using the multi-string Burrows-Wheeler transform. All data can be used without restriction.

引用

页码：4211 / 4216

页数：6