Soy Glycinin Contains a Functional Inhibitory Sequence against Muscle-Atrophy-Associated Ubiquitin Ligase Cbl-b

被引:27
作者
Abe, Tomoki [1 ]
Kohno, Shohei [1 ]
Yama, Tomonari [1 ]
Ochi, Arisa [1 ]
Suto, Takuro [1 ]
Hirasaka, Katsuya [1 ]
Ohno, Ayako [1 ]
Teshima-Kondo, Shigetada [1 ]
Okumura, Yuushi [1 ]
Oarada, Motoko [2 ]
Choi, Inho [3 ]
Mukai, Rie [4 ]
Terao, Junji [4 ]
Nikawa, Takeshi [1 ]
机构
[1] Univ Tokushima, Grad Sch, Inst Hlth Biosci, Dept Nutr Physiol, Tokushima 7708503, Japan
[2] Chiba Univ, Med Mycol Res Ctr, Chiba 2608673, Japan
[3] Yonsei Univ, Inst Biomat, Div Biol Sci & Technol, Coll Sci & Technol, Wonju 220710, South Korea
[4] Univ Tokushima, Inst Hlth Biosci, Dept Food Sci, Tokushima 7708503, Japan
关键词
SKELETAL-MUSCLE; GROWTH-HORMONE; PROTEIN;
D O I
10.1155/2013/907565
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background. Unloading stress induces skeletal muscle atrophy. We have reported that Cbl-b ubiquitin ligase is a master regulator of unloading-associated muscle atrophy. The present study was designed to elucidate whether dietary soy glycinin protein prevents denervation-mediated muscle atrophy, based on the presence of inhibitory peptides against Cbl-b ubiquitin ligase in soy glycinin protein. Methods. Mice were fed either 20% casein diet, 20% soy protein isolate diet, 10% glycinin diet containing 10% casein, or 20% glycinin diet. One week later, the right sciatic nerve was cut. The wet weight, cross sectional area (CSA), IGF-1 signaling, and atrogene expression in hindlimb muscles were examined at 1, 3, 3.5, or 4 days after denervation. Results. 20% soy glycinin diet significantly prevented denervation-induced decreases in muscle wet weight and myofiber CSA. Furthermore, dietary soy protein inhibited denervation-induced ubiquitination and degradation of IRS-1 in tibialis anterior muscle. Dietary soy glycinin partially suppressed the denervation-mediated expression of atrogenes, such as MAFbx/atrogin-1 and MuRF-1, through the protection of IGF-1 signaling estimated by phosphorylation of Akt-1. Conclusions. Soy glycinin contains a functional inhibitory sequence against muscle-atrophy-associated ubiquitin ligase Cbl-b. Dietary soy glycinin protein significantly prevented muscle atrophy after denervation in mice.
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页数:11
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