Chromosome-centric approach to overcoming bottlenecks in the Human Proteome Project

被引：1

作者：

Archakov, Alexander ^{[1
]}

Zgoda, Victor ^{[1
]}

Kopylov, Arthur ^{[1
]}

Naryzhny, Stanislav ^{[1
,2
]}

Chernobrovkin, Alexey ^{[1
]}

Ponomarenko, Elena ^{[1
]}

Lisitsa, Andrey ^{[1
]}

机构：

[1] Russian Acad Med Sci, Orekhovich Inst Biomed Chem, Moscow 119121, Russia

[2] Petersburg Nucl Phys Inst, Gatchina, Leningrad Distr, Russia

来源：

EXPERT REVIEW OF PROTEOMICS | 2012年 / 9卷 / 06期

关键词：

Human Genome Project; Human Proteome Project; limit of detection; selected reaction monitoring; HUMAN PLASMA PROTEOME; MASS-SPECTROMETRY; PILOT PHASE; PROTEINS; PEPTIDE; SENSITIVITY; IDENTIFICATION; PHOSPHORYLATION; QUANTIFICATION; TRANSCRIPTOME;

D O I：

10.1586/EPR.12.54

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The international Human Proteome Project (HPP), a logical continuation of the Human Genome Project, was launched on 23 September 2010 in Sydney, Australia. In accordance with the genecentric approach, the goals of the HPP are to prepare an inventory of all human proteins and decipher the network of cellular protein interactions. The greater complexity of the proteome in comparison to the genome gives rise to three bottlenecks in the implementation of the HPP. The main bottleneck is the insufficient sensitivity of proteomic technologies, hampering the detection of proteins with low- and ultra-low copy numbers. The second bottleneck is related to poor reproducibility of proteomic methods and the lack of a so-called 'gold' standard. The last bottleneck is the dynamic nature of the proteome: its instability over time. The authors here discuss approaches to overcome these bottlenecks in order to improve the success of the HPP.

引用

页码：667 / 676

页数：10

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