Anti-colon cancer effect of matrix protein gene therapy with nanoparticles

被引：2

作者：

Wang, Bilan ^{[1
,2
]}

Yang, Daoke ^{[3
]}

Shen, Yangmei ^{[2
,4
]}

机构：

[1] Sichuan Univ, West China Second Univ Hosp, Dept Pharm, Chengdu 610041, Sichuan, Peoples R China

[2] Sichuan Univ, West China Univ Hosp 2, Minist Educ, Key Lab Birth Defects & Related Dis Women & Child, Chengdu 610041, Sichuan, Peoples R China

[3] Zhengzhou Univ, Affiliated Hosp 1, Tumor Hosp, Zhengzhou 450052, Henan, Peoples R China

[4] Sichuan Univ, West China Univ Hosp 2, Dept Pathol, Chengdu 610041, Sichuan, Peoples R China

来源：

POLYMER | 2019年 / 170卷

关键词：

Matrix protein; Cancer; PEG-PCL-PEG; VESICULAR STOMATITIS-VIRUS; CATIONIC LIPIDS; TOXICITY; CELLS; SUPPRESSION; INHIBITION; DELIVERY; POTENCY; SIRNA;

D O I：

10.1016/j.polymer.2019.03.023

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The matrix protein (MP) is the core structure of the oncolytic vesicular stomatitis virus (VSV) and it played a vital role during the process of VSV infection and corresponding cytopathic effects in cancer treatment. In this study, a novel delivery synthesized by PEG-PCL-PEG and DOTAP (PPPD) was applied to encapsulate MP plasmids to derive the advantage and overcome the drawback of VSV platform in the treatment of colon cancer. The MP/DPPP micelles were stable in physiological solution, with an average diameter of 102 nm and zeta potential of 5.4 mV. This delivery system has a high encapsulation rate of 96% and transfection efficiency of 36%. A prominent anti-cancer activity of MP/DPPP was found both in Ct26 cells and animal models. The mechanisms of antitumor were further explored in this research. Our results indicated that MP/DPPP micelles were promising agents for future clinical application in the treatment of colon cancer.

引用

页码：148 / 156

页数：9

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