Photothermally-induced disordered patterns of corneal collagen revealed by SHG imaging

被引：130

作者：

Matteini, Paolo ^{[1
]}

Ratto, Fulvio ^{[1
]}

Rossi, Francesca ^{[1
]}

Cicchi, Riccardo ^{[2
]}

Stringari, Chiara ^{[2
]}

Kapsokalyvas, Dimitrios ^{[2
]}

Pavone, Francesco S. ^{[2
]}

Pini, Roberto ^{[1
]}

机构：

[1] CNR, Ist Fis Applicata Nello Carrara, I-50019 Sesto Fiorentino, Italy

[2] LENS, European Lab Nonlinear Spect, I-50019 Sesto Fiorentino, Italy

来源：

OPTICS EXPRESS | 2009年 / 17卷 / 06期

关键词：

2ND-HARMONIC GENERATION MICROSCOPY; FLUORESCENCE; TRANSITIONS; FIBRILS; TISSUES; SCLERA;

D O I：

10.1364/OE.17.004868

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The loss of organization of the corneal collagen lattice induced by photothermal effects was analyzed by using second-harmonic generation (SHG) imaging. Porcine cornea samples were treated with low-power laser irradiation in order to get localized areas of tissue disorganization. The disorder induced within the irradiated area of corneal stroma was quantified by means of Discrete Fourier Transform, auto-correlation and entropy analyses of the SHG images. Polarization modulated SHG measurements allowed to probe the changes in the structural anisotropy of sub-micron hierarchical levels of the stromal collagen. Our results emphasize the great potential of the SHG imaging to detect subtle modifications in the collagen assembly. The proposed analytical methods may be used to track several genetic, pathologic, accidental or surgical-induced disorder states of biological tissues. (C) 2008 Optical Society of America

引用

页码：4868 / 4878

页数：11

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