UV-Fixed-Thick-Blotch Preparation Improves Sensitivity of Auramine Staining

被引：8

作者：

Cejudo-Garcia, M. A. ^{[1
]}

Munoz-Davila, M. J. ^{[1
]}

Martinez-Lirola, M. J. ^{[1
]}

机构：

[1] Biotechnol Clin Management Unit, Microbiol Lab, Almeria, Spain

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2013年 / 51卷 / 10期

关键词：

TUBERCULOSIS; MICROSCOPY; DIAGNOSIS;

D O I：

10.1128/JCM.00974-13

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We describe here a very simple modification of the auramine staining procedure based on preparation of a UV-fixed thick blotch which allowed us to reach an overall sensitivity of 0.82 (592 acid-fast bacillus [AFB]-positive specimens/722 initial respiratory specimens with positive mycobacterial culture) and sensitivities of 0.93 (526 AFB-positive specimens/564 culture-positive specimens) for Mycobacterium tuberculosis complex and 0.42 (66 AFB-positive specimens/158 culture-positive specimens) for nontuberculous mycobacteria.

引用

页码：3400 / 3402

页数：3

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