Autoantibodies against oxidized LDL correlate with serum concentrations of ceruloplasmin in patients with cardiovascular disease

Background: Ceruloplasmin (CP) has been suggested to play a role in the oxidative modification of LDL. The correlation between autoantibodies against oxidized LDL (anti-oxLDL), markers of oxidative stress, and the concentration of CP has not been previously investigated. We examined the status of these parameters in patients with myocardial infarction (MI) and stable angina. Methods: Blood samples were collected from patients with MI (n=56), stable angina (n=96) and from healthy controls (n=109). Levels of CP and copper were determined using turbidimetry and atomic absorption spectrophotometry, respectively. Levels of anti-oxLDL were obtained by ELISA. Results: Serum concentrations of CP, copper and anti-oxLDL were significantly higher in both groups of patients than those in controls. Among patient groups, concentrations of CP and copper were not significantly different, however, the concentrations of anti-oxLDL were higher in MI patients than that in angina (P=0.001), and were greatly influenced by underlying conditions such as diabetes mellitus, hypertension and smoking. Significant positive correlation was observed between serum concentrations of both CP and copper and the concentrations of anti-oxLDL in both patient groups but not in controls. Conclusions: High concentrations of anti-oxLDL suggest an increase in oxidative stress that would contribute to disease severity. The observed correlation of CP with anti-oxLDL may suggest a possible pro-oxidative activity of CP in patients with cardiovascular disease. (c) 2005 Elsevier B.V. All rights reserved.