Salicylic Acid Improves Root Antioxidant Defense System and Total Antioxidant Capacities of Flax Subjected to Cadmium

被引:20
作者
Belkadhi, Aicha [1 ,2 ]
De Haro, Antonio [2 ]
Soengas, Pilar [3 ]
Obregon, Sara [2 ]
Cartea, Maria Elena [3 ]
Djebali, Wahbi [1 ]
Chaibi, Wided [1 ]
机构
[1] Fac Sci Tunis, Unite Rech Physiol & Biochim Tolerance Plantes Au, Dept Biol, Tunis 1060, Tunisia
[2] Spanish Council Sci Res CSIC, Inst Sustainable Agr, Dept Agron & Plant Breeding, Cordoba, Spain
[3] Spanish Council Sci Res CSIC, Dept Plant Genet, Pontevedra, Spain
关键词
INDUCED OXIDATIVE STRESS; TOMATO LYCOPERSICON-ESCULENTUM; PHOTOSYNTHETIC ACTIVITY; EXOGENOUS TREATMENT; REDOX HOMEOSTASIS; CATALASE ACTIVITY; NITRIC-OXIDE; TOXICITY; GROWTH; L;
D O I
10.1089/omi.2013.0030
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Cadmium (Cd) disrupts the normal growth and development of plants, depending on their tolerance to this toxic element. The present study was focused on the impacts of exogenous salicylic acid (SA) on the response and regulation of the antioxidant defense system and membrane lipids to 16-day-old flax plantlets under Cd stress. Exposure of flax to high Cd concentrations led to strong inhibition of root growth and enhanced lipid peroxides, membrane permeability, protein oxidation, and hydrogen peroxide (H2O2) production to varying degrees. Concomitantly, activities of the antioxidant enzymes catalase (CAT, EC 1.11.1.6), guaicol peroxydase (GPX, EC 1.11.1.7), ascorbate peroxydase (APX, EC 1.11.1.11), and superoxide dismutase (SOD, EC 1.15.1.1), and the total antioxidant capacities (2,2'-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and ferric reducing antioxidant power (FRAP)) were significantly altered by Cd. In contrast, exogenous SA greatly reduced the toxic effects of Cd on the root growth, antioxidant system, and membrane lipid content. The Cd-treated plantlets pre-soaked with SA exhibited less lipid and protein oxidation and membrane alteration, as well as a high level of total antioxidant capacities and increased activities of antioxidant enzymes except of CAT. These results may suggest that SA plays an important role in triggering the root antioxidant system, thereby preventing membrane damage as well as the denaturation of its components.
引用
收藏
页码:398 / 406
页数:9
相关论文
共 72 条
[1]  
Aebi H, 1984, Methods Enzymol, V105, P121
[2]   Exogenous treatment with Salicylic acid leads to increased antioxidant capacity in leaves of barley plants exposed to Paraquat [J].
Ananieva, EA ;
Christov, KN ;
Popova, LP .
JOURNAL OF PLANT PHYSIOLOGY, 2004, 161 (03) :319-328
[3]   Reactive oxygen species: Metabolism, oxidative stress, and signal transduction [J].
Apel, K ;
Hirt, H .
ANNUAL REVIEW OF PLANT BIOLOGY, 2004, 55 :373-399
[4]   Response of antioxidant defence system in soybean nodules and roots subjected to cadmium stress [J].
Balestrasse, KB ;
Gardey, L ;
Gallego, SM ;
Tomaro, ML .
AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY, 2001, 28 (06) :497-504
[5]   SUPEROXIDE DISMUTASE - IMPROVED ASSAYS AND AN ASSAY APPLICABLE TO ACRYLAMIDE GELS [J].
BEAUCHAM.C ;
FRIDOVIC.I .
ANALYTICAL BIOCHEMISTRY, 1971, 44 (01) :276-&
[6]   Effects of exogenous salicylic acid pre-treatment on cadmium toxicity and leaf lipid content in Linum usitatissimum L. [J].
Belkhadi, A. ;
Hediji, H. ;
Abbes, Z. ;
Nouairi, I. ;
Barhoumi, Z. ;
Zarrouk, M. ;
Chaibi, W. ;
Djebali, W. .
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2010, 73 (05) :1004-1011
[7]   Cadmium effects on lipid metabolism of rape (Brassica napus L.) [J].
Ben Youssef, N ;
Nouairi, I ;
Ben Temime, S ;
Taamalli, W ;
Zarrouk, M ;
Ghorbal, MH ;
Daoud, DB .
COMPTES RENDUS BIOLOGIES, 2005, 328 (08) :745-757
[8]   The ferric reducing ability of plasma (FRAP) as a measure of ''antioxidant power'': The FRAP assay [J].
Benzie, IFF ;
Strain, JJ .
ANALYTICAL BIOCHEMISTRY, 1996, 239 (01) :70-76
[9]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[10]  
BRAND-WILLIAMS W, 1995, FOOD SCI TECHNOL-LEB, V28, P25