Effect of temperature on continuous fermentative hydrogen production from Laminaria japonica by anaerobic mixed cultures

被引:34
作者
Shi, Xueqing [1 ]
Kim, Dong-Hoon [2 ]
Shin, Hang-Sik [3 ]
Jung, Kyung-Won [4 ]
机构
[1] Natl Univ Singapore, Dept Civil & Environm Engn, Ctr Water Res, Singapore 119077, Singapore
[2] Korea Inst Energy Res, Bioenergy Res Ctr, Taejon 305343, South Korea
[3] Korea Adv Inst Sci & Technol, Dept Civil & Environm Engn, Taejon 305701, South Korea
[4] Korea Inst Sci & Technol, Ctr Environm Technol Res, Seoul 130650, South Korea
基金
新加坡国家研究基金会;
关键词
Dark fermentative hydrogen production; Laminaria japonica; Mixed cultures; Temperature; Total cellulase activity; TRICHODERMA-REESEI; LIGNOCELLULOSIC BIOMASS; BIOHYDROGEN PRODUCTION; CORNSTALK WASTES; PRETREATMENT; CELLULASES; CONVERSION;
D O I
10.1016/j.biortech.2013.06.107
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
The temperature effect on continuous dark fermentative hydrogen production from non-pretreated Laminaria japonica was investigated in the present study. In a preliminary step, the fermentors were continuously operated as an inoculation process at three different temperatures, 35, 50 and 65 degrees C, to respectively represent mesophilic, thermophilic, and hyperthermophilic conditions. An optimization process was subsequently conducted with a range of organic loading rate (OLR) and cultivation pH. Among the various operation conditions, the maximum H-2 yield, 61.3 +/- 2.0 mL H-2/g TS, was observed under a mesophilic condition at OLR of 3.4 g COD/L/d and pH 5.5. From a PCR-DGGE analysis, it was found that an increase of temperature can reduce the microbial diversity and change the predominant species. Finally, total cellulase activity was measured, to investigate the effect of temperature on hydrolysis of L. japonica. The highest cellulase activity was 0.19 +/- 0.02 FPU/mL, observed at 35 degrees C, coinciding with the maximum H-2 yield. (c) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:225 / 231
页数:7
相关论文
共 28 条
[1]   Hydrogen production potentials and fermentative characteristics of various substrates with different heat-pretreated natural microflora [J].
Akutsu, Yohei ;
Lee, Dong-Yeol ;
Li, Yu-You ;
Noike, Tatsuya .
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 2009, 34 (13) :5365-5372
[2]  
[Anonymous], 2006, STANDARD METHODS EXA, DOI DOI 10.5860/CHOICE.37-2792
[3]   Effect of substrate concentration on fermentative hydrogen production from sweet sorghum extract [J].
Antonopoulou, Georgia ;
Gavala, Hariklia N. ;
Skiadas, Ioannis V. ;
Lyberatos, Gerasimos .
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 2011, 36 (08) :4843-4851
[4]   Enhanced cellulosic hydrogen production from lime-treated cornstalk wastes using thermophilic anaerobic microflora [J].
Cao, Guang-Li ;
Guo, Wan-Qian ;
Wang, Ai-Jie ;
Zhao, Lei ;
Xu, Cheng-Jiao ;
Zhao, Qing-long ;
Ren, Nan-Qi .
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 2012, 37 (17) :13161-13166
[5]   Efficient conversion of wheat straw wastes into biohydrogen gas by cow dung compost [J].
Fan, YT ;
Zhang, YH ;
Zhang, SF ;
Hou, HW ;
Ren, BZ .
BIORESOURCE TECHNOLOGY, 2006, 97 (03) :500-505
[6]   MEASUREMENT OF CELLULASE ACTIVITIES [J].
GHOSE, TK .
PURE AND APPLIED CHEMISTRY, 1987, 59 (02) :257-268
[7]   Continuous fermentative hydrogen and methane production from Laminaria japonica using a two-stage fermentation system with recycling of methane fermented affluent [J].
Jung, Kyung-Won ;
Kim, Dong-Hoon ;
Shin, Hang-Sik .
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 2012, 37 (20) :15648-15657
[8]   Fermentative hydrogen production from Laminaria japonica and optimization of thermal pretreatment conditions [J].
Jung, Kyung-Won ;
Kim, Dong-Hoon ;
Shin, Hang-Sik .
BIORESOURCE TECHNOLOGY, 2011, 102 (03) :2745-2750
[9]   Thermophilic fermentative hydrogen production from various carbon sources by anaerobic mixed cultures [J].
Kim, Dong-Hoon ;
Kim, Mi-Sun .
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 2012, 37 (02) :2021-2027
[10]   Sewage sludge addition to food waste synergistically enhances hydrogen fermentation performance [J].
Kim, Dong-Hoon ;
Kim, Sang-Hyoun ;
Kim, Hyun-Woo ;
Kim, Mi-Sun ;
Shin, Hang-Sik .
BIORESOURCE TECHNOLOGY, 2011, 102 (18) :8501-8506