Seed train development for the fermentation of bagasse from sweet sorghum and sugarcane using a simplified fermentation process

被引：34

作者：

Geddes, C. C. ^{[1
,2
]}

Mullinnix, M. T. ^{[3
]}

Nieves, I. U. ^{[3
]}

Hoffman, R. W. ^{[3
]}

Sagues, W. J. ^{[3
]}

York, S. W. ^{[1
]}

Shanmugam, K. T. ^{[1
]}

Erickson, J. E. ^{[4
]}

Vermerris, W. E. ^{[5
,6
]}

Ingram, L. O. ^{[1
,3
]}

机构：

[1] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA

[2] Novozymes, CE Geddes, Franklinton, NC 27525 USA

[3] Univ Florida, Stan Mayfield Biorefinery Pilot Plant, Perry, FL 32347 USA

[4] Univ Florida, Dept Agron, Gainesville, FL 32611 USA

[5] Univ Florida, Dept Agron, Gainesville, FL 32610 USA

[6] Univ Florida, Genet Inst, Gainesville, FL 32610 USA

来源：

BIORESOURCE TECHNOLOGY | 2013年 / 128卷

关键词：

Biomass; Lignocellulose; SScF; L plus SScF; Ethanol; Xylose; ETHANOL-PRODUCTION; INHIBITORS; PRETREATMENT; DETOXIFICATION; LIGNOCELLULOSE; HYDROLYSATE; TOLERANCE; STRAIN; FUEL; SACCHARIFICATION;

D O I：

10.1016/j.biortech.2012.09.121

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

A process was developed for seed culture expansion (3.6 million-fold) using 5% of the hemicellulose hydrolysate from dilute acid pretreatment as the sole organic nutrient and source of sugar. Hydrolysate used for seed growth was neutralized with ammonia and combined with 1.0 mM sodium metabisulfite immediately before inoculation. This seed protocol was tested with phosphoric acid pretreated sugarcane and sweet sorghum bagasse using a simplified process with co-fermentation of fiber, pentoses, and hexoses in a single vessel (SScF). A 6 h liquefaction (L) step improved mixing prior to inoculation. Fermentations (L + SScF process) were completed in 72 h with high yields (>80 gal/US ton). Ethanol titers for this L + SScF process ranged from 24 g/L to 32 g/L, and were limited by the bagasse concentration (10% dry matter). (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：716 / 724

页数：9

共 39 条

[1] Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae [J].

Almeida, Jodo R. M. ;

Modig, Tobias ;

Petersson, Anneli ;

Hahn-Hagerdal, Barbel ;

Liden, Gunnar ;

Gorwa-Grauslund, Marie F. .

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2007, 82 (04) :340-349

[2] Improving the fermentability of enzymatic hydrolysates of lignocellulose through chemical in-situ detoxification with reducing agents [J].

Alriksson, Bjorn ;

Cavka, Adnan ;

Jonsson, Leif J. .

BIORESOURCE TECHNOLOGY, 2011, 102 (02) :1254-1263

[3] Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review [J].

Alvira, P. ;

Tomas-Pejo, E. ;

Ballesteros, M. ;

Negro, M. J. .

BIORESOURCE TECHNOLOGY, 2010, 101 (13) :4851-4861

[4] EFFICIENT FERMENTATION OF PINUS SP ACID HYDROLYSATES BY AN ETHANOLOGENIC STRAIN OF ESCHERICHIA-COLI [J].

BARBOSA, MDS ;

BECK, MJ ;

FEIN, JE ;

POTTS, D ;

INGRAM, LO .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (04) :1382-1384

[5] Multifaceted characterization of cell wall decomposition products formed during ammonia fiber expansion (AFEX) and dilute acid based pretreatments [J].

Chundawat, Shishir P. S. ;

Vismeh, Ramin ;

Sharma, Lekh N. ;

Humpula, James F. ;

Sousa, Leonardo da Costa ;

Chambliss, C. Kevin ;

Jones, A. Daniel ;

Balan, Venkatesh ;

Dale, Bruce E. .

BIORESOURCE TECHNOLOGY, 2010, 101 (21) :8429-8438

[6]

FONTANA JD, 1984, BIOTECHNOL BIOENG, P175

[7] Pretreatment of lignocellulosic materials for efficient bioethanol production [J].

Galbe, Mats ;

Zacchi, Guido .

BIOFUELS, 2007, 108 :41-65

[8] Simplified process for ethanol production from sugarcane bagasse using hydrolysate-resistant Escherichia coli strain MM160 [J].

Geddes, C. C. ;

Mullinnix, M. T. ;

Nieves, I. U. ;

Peterson, J. J. ;

Hoffman, R. W. ;

York, S. W. ;

Yomano, L. P. ;

Miller, E. N. ;

Shanmugam, K. T. ;

Ingram, L. O. .

BIORESOURCE TECHNOLOGY, 2011, 102 (03) :2702-2711

[9] Advances in ethanol production [J].

Geddes, Claudia C. ;

Nieves, Ismael U. ;

Ingram, Lonnie O. .

CURRENT OPINION IN BIOTECHNOLOGY, 2011, 22 (03) :312-319

[10] Removal of enzymatic and fermentation inhibitory compounds from biomass slurries for enhanced biorefinery process efficiencies [J].

Gurram, Raghu N. ;

Datta, Saurav ;

Lin, Yupo J. ;

Snyder, Seth W. ;

Menkhaus, Todd J. .

BIORESOURCE TECHNOLOGY, 2011, 102 (17) :7850-7859

← 1 2 3 4 →