Crystallographic analysis of FAD-dependent glucose dehydrogenase

被引：2
|
作者：
Komori, Hirofumi [1 ,2 ]
Inaka, Koji [3 ]
Furubayashi, Naoki [3 ]
Honda, Michinari [4 ]
Higuchi, Yoshiki [2 ,5 ]
机构：
[1] Kagawa Univ, Fac Educ, Takamatsu, Kagawa 7608522, Japan
[2] RIKEN, SPring Ctr 8, Mikazuki, Hyogo 6795198, Japan
[3] Maruwa Foods & Biosci Inc, Nara 6391123, Japan
[4] Ikeda Tohka Ind Co Ltd, Fukuyama, Hiroshima 7210956, Japan
[5] Univ Hyogo, Dept Life Sci, Kamigori, Hyogo 6781297, Japan
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2015年 / 71卷
基金：
日本学术振兴会;
关键词：
glucose dehydrogenase; FAD; glucose sensor; ANGSTROM RESOLUTION;
D O I：
10.1107/S2053230X15010742
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An FAD-dependent glucose dehydrogenase (GDH) from Aspergillus terreus was purified and crystallized at 293K using the sitting-drop vapour-diffusion method. A data set was collected to a resolution of 1.6 angstrom from a single crystal at 100K using a rotating-anode X-ray source. The crystal belonged to space group P2(1), with unit-cell parameters a = 56.56, b = 135.74, c = 74.13 angstrom, = 90.37 degrees. The asymmetric unit contained two molecules of GDH. The Matthews coefficient was calculated to be 2.2 angstrom(3)Da(-1) and the solvent content was estimated to be 44%.
引用
收藏
页码：1017 / 1019
页数：3
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