Effect of pore structure of SBA-15 on immobilization of hemoglobin

被引:8
作者
Zhou, Li-Hui [1 ,2 ]
Tao, Ying-Hua [1 ,2 ]
Hu, Jun [1 ,2 ]
Han, Xia [1 ,2 ]
Liu, Hong-Lai [1 ,2 ]
Hu, Ying [1 ,2 ]
机构
[1] E China Univ Sci & Technol, State Key Lab Chem Engn, Shanghai 200237, Peoples R China
[2] E China Univ Sci & Technol, Dept Chem, Shanghai 200237, Peoples R China
基金
中国国家自然科学基金;
关键词
SBA-15; Mesoporous structure; Hemoglobin; Immobilization; Binding; Leaching;
D O I
10.1007/s10934-007-9146-y
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Immobilization of hemoglobin (Hb) in SBA-15 with various pore sizes by physical adsorption was studied. The structure changes of mesoporous SBA-15 before and after the immobilization of Hb were characterized by N-2 adsorption isotherms, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and Ultraviolet-visible spectroscopy (UV). The results indicate that SBA-15 is a good support for the immobilization of Hb due to its regular structure, large pore diameter, and high surface area. After immobilization of Hb, the regular structure of SBA-15 is still kept, but the pore diameter, pore volume and surface area decrease. With the increase of pore size, the binding amount and leaching amount of Hb increase. There is a maximum binding amount of Hb up to 355.2 mg/g (SBA-15) when pore size is 8.9 nm. It is suggested that the immobilization of Hb depends significantly on the pore size of SBA-15.
引用
收藏
页码:653 / 659
页数:7
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