Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins

被引：28

作者：

Oliveira, R ^{[1
]}

Marques, F ^{[1
]}

Azeredo, J ^{[1
]}

机构：

[1] Univ Minho, IBQF, Ctr Engn Biol, P-4700 Braga, Portugal

来源：

BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 06期

关键词：

differential precipitation; polysaccharides; proteins;

D O I：

10.1023/A:1008954301470

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The objective was to obtain the polysaccharide fraction present in the extracted polymeric matrix of a biofilm by differential precipitation of the protein content. This was tried by pH adjustment and by addition of trichloroacetic acid (TCA). The best yield of protein precipitation was with 20% (w/v) TCA, with more than 97% of the protein removed and without affecting the amount of polysaccharides in solution.

引用

页码：391 / 393

页数：3