Orthogonal supramolecular protein assembly on patterned bifunctional surfaces

被引：4
作者：
Wasserberg, D. [1 ,2 ,3 ,4 ,5 ]
Cabanas-Danes, J. [1 ,2 ,3 ]
Subramaniam, V. [2 ,4 ,5 ,6 ]
Huskens, J. [3 ]
Jonkheijm, P. [1 ,2 ,3 ]
机构：
[1] Univ Twente, MIRA Biomed Technol, Bioinspired Mol Engn Lab, POB 217, NL-7500 AE Enschede, Netherlands
[2] Univ Twente, Tech Med Inst, POB 217, NL-7500 AE Enschede, Netherlands
[3] Univ Twente, MESA Inst Nanotechnol, Mol Nanofabricat Grp, NL-7500 AE Enschede, Netherlands
[4] Univ Twente, MESA Inst Nanotechnol, Nanobiophys Grp, NL-7500 AE Enschede, Netherlands
[5] Univ Twente, MIRA Biomed Technol, NL-7500 AE Enschede, Netherlands
[6] Free Univ Amsterdam, Boelelaan 1105, NL-1081 HV Amsterdam, Netherlands
来源：
CHEMICAL COMMUNICATIONS | 2018年 / 54卷 / 13期
关键词：
MOLECULAR PRINTBOARDS; FUNCTIONAL PROTEINS; MONOLAYERS; IMMOBILIZATION; NANOSTRUCTURES; CONSTRUCTION; DIMERIZATION; MICROARRAYS; NANOSCALE; GOLD;
D O I：
10.1039/c7cc09808a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report successful and selective dual protein assembly on patterned bifunctional beta CD-Ni(II) NTA surfaces, using red fluorescent protein variants with hexahistidine-tags and teal fluorescent protein variants conjugated with a peptide containing three adamantyl groups. We show that dual protein patterns can only be assembled, when opposing supramolecular interactions have been optimized and nonspecific interactions have been sufficiently suppressed.
引用
收藏
页码：1615 / 1618
页数：4
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