Study on Synthesis and Characterization of a Fluorescence Probe and Its Application for Determination of Superoxide Anion Radical

被引：3
|
作者：
Zhao, Yongqiang [1 ,2 ]
Lin, Hong [1 ]
Li, Laihao [2 ]
Yang, Xianqing [2 ]
Hao, Shuxian [2 ]
Cen, Jianwei [2 ]
Wei, Ya [2 ]
Qi, Bo [2 ]
Huang, Hui [2 ]
机构：
[1] Ocean Univ China, Food Safety Lab, Qingdao, Peoples R China
[2] Chinese Acad Fishery Sci, Natl R&D Ctr Aquat Prod Proc, South China Sea Fisheries Res Inst, Guangzhou, Guangdong, Peoples R China
来源：
BIOTECHNOLOGY, CHEMICAL AND MATERIALS ENGINEERING, PTS 1-3 | 2012年 / 393-395卷
关键词：
synthesis; characterization; fluorescence probe; superoxide anion radical; MECHANISMS; ASSAY;
D O I：
10.4028/www.scientific.net/AMR.393-395.1483
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A novel Schiff base, 2-(Salicylideneamino)pyridine (2-SAP) synthesized by 2-aminopyridine and salicylaldehyde was used as a fluorescence probe to determine superoxide anion radical (O-2(center dot-)) in this study. The synthetic product was characterized by IR, H-1-NMR and GC-MS methods. A fluorescence quenching reaction occurs between 2-SAP and O-2(center dot-), and the fluorescence intensity decreased significantly. The effect of pyrogallol concentration (2.0x10(-5) M to1.0x10(-3) M) was discussed in this study. In the range from 2.0x10(-5) M to 1.0x10(-4) M, relative fluorescence intensity (y) and pyrogallol concentration (x) shown a good linear relationship,.y=37.801x+76.854, r=0.993. Based on this phenomenon, the O-2(center dot-) generated by pyrogallol autoxidation could be determined simply with high sensitivity.
引用
收藏
页码：1483 / +
页数：2
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