Development of a lion-specific interferon-gamma assay

The ongoing spread of bovine tuberculosis (BTB) in African free-ranging lion populations, for example in the Kruger National Park, raises the need for diagnostic assays for BIB in lions. These, in addition, would be highly relevant for zoological gardens worldwide that want to determine the BTB status of their lions, e.g. for translocations. The present study concerns the development of a lion-specific IFN-gamma assay, following the production and characterization of monoclonal antibodies specific for lion interferon-gamma (IFN-gamma). Recombinant lion IFN-gamma (rLIFN-gamma) was produced in mammalian cells and used to immunize mice to establish hybridoma cell lines producing monoclonal antibodies. These were used to develop a sensitive, lion IFN-gamma-specific capture ELISA, able to detect rLIFN-gamma to the level of 160 pg/ml. Recognition of native lion IFN-gamma was shown in an initial assessment of supernatants of mitogen stimulated whole blood cultures of 11 known BIB-negative lions. In conclusion, the capture ELISA shows potential as a diagnostic assay for bovine tuberculosis in lions. Preliminary results also indicate the possible use of the test for other (feline) species. (C) 2012 Elsevier B.V. All rights reserved.