Radio-protective effect of catalpol in cultured cells and mice

被引：29

作者：

Chen, Chengjie ^{[1
,2
,3
]}

Chen, Zhe ^{[1
]}

Xu, Fang ^{[3
]}

Zhu, Chao ^{[3
]}

Fang, Fanfu ^{[1
]}

Shu, Shi ^{[1
]}

Li, Min ^{[2
]}

Ling, Changquan ^{[1
]}

机构：

[1] Changhai Hosp, Dept Tradit Chinese Med, Shanghai 200433, Peoples R China

[2] Second Mil Med Univ, Dept Naval Med, Shanghai 200433, Peoples R China

[3] Airforce Hangzhou Aviat Med Appraisal & Training, Hangzhou 310007, Zhejiang, Peoples R China

来源：

JOURNAL OF RADIATION RESEARCH | 2013年 / 54卷 / 01期

关键词：

catalpol; radioprotection; apoptosis; reactive oxygen species; OXIDATIVE DNA-DAMAGE; STRESS; RADIATION; APOPTOSIS; CARCINOGENESIS; ASSAY;

D O I：

10.1093/jrr/rrs080

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ionizing radiation can induce DNA damage and cell death by generating reactive oxygen species (ROS). The objective of this study was to investigate the radio-protective effect of catalpol (a main bioactive component in the traditional Chinese Rehmannia) on irradiated cells and mice. We found that treating cells with catalpol (25-100 mu g/ml) before irradiation could significantly inhibit ionizing radiation (IR)-induced human lymphocyte AHH-1 cells apoptosis and increase cells viability in vitro. At the same time our study also showed that catalpol (25-100 mg/kg) reduced morphological damage of the gastrointestinal tract by 15.6%, 33.3% and 44.4%, respectively compared with the radiation-induced group, decreased plasma malondialdehyde (MDA) intestinal 8-hydroxydeoxyguanosine (8-OHdG) levels and increased plasma endogenous antioxidants and peripheral white blood cells and platelets in vivo. These results suggest that catalpol possesses notable radio-protective activity, which might be related to its effect of reducing ROS.

引用

页码：76 / 82

页数：7

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